Under general supervision of Principal Investigator, and in accordance with established hospital policies and procedures, conducts experiments to identify new therapeutic strategies in pediatric and adult cancers (previous studies by the lab have led to FDA approval of many new drugs for cancer). This research assistant position is cell culture intensive and uses viability, protein (immunoblotting), and flow cytometry analytic techniques to evaluate tumor cell cultures after treatment with novel therapies. The position also includes responsibility (along with other lab members) for genome sequencing and other high-throughput analytics to identify therapeutically-relevant biologic vulnerabilities in cancer. These studies are coupled with functional validations (gene knockdowns and overexpression) to confirm and understand the therapeutic opportunities. Ideally, the research assistant will look forward to preparing manuscripts for publication in high-impact science and medicine journals, so strong writing ability is a plus. The research assistant will also assist in the training of new students and postdoctoral fellows in standard methods of molecular biology and cell culture. Perfect opportunity for someone planning to attend medical or graduate school in 2-3 years!
This laboratory is primarily interested in the biology and therapeutics of cancer.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Contributes to genomic and proteomic screens to identify novel therapeutic targets in human cancer. Performs tumor cell cultures and evaluates effects of treating tumor cultures with novel therapies. Carries out standard methods of molecular biology including: Isolation of various cell fractions from blood, bone marrow and solid tumor specimens; immunoprecipitation and western blotting, Polymerase chain reaction DNA and cDNA amplification; DNA cloning in phage and plasmids; next-generation sequencing; fluorescence in situ hybridization;
Records data, summarizes and analyzes results using basic statistics.
Reports on progress of work at weekly laboratory seminars.
Prepares laboratory reagents, chemicals, instruments and equipment.
Maintains supplies inventory in accordance with department procedures.
Functions as a key team member in drafting and otherwise preparing high-impact publications.
Helps train new students and postdoctoral fellows in standard methods of molecular biology.
Maintains established departmental policies, procedures and objectives, including the areas of quality assurance, safety, environmental affairs and infection control.
Performs all other duties and responsibilities as directed.
Bachelor of Science or Bachelor of Arts degree in a biological/physical science required.
Some laboratory experience desired. Undergraduate coursework or projects may be considered as appropriate experience, and may be credited upon hire if project(s) directly related to position. Undergraduate experience with Creative Writing (or other courses that focus on writing skills, such as critical or analytical writing) is a plus.
Ability to carry out procedures in a coordinated multi-step manner with appropriate consultation.
Ability to methodically record data and to analyze data using basic statistics.
Ability to contribute to manuscript drafts: specifically, ability to communicate complex science concepts in written form, using correct grammar and syntax.
Ability to work under general supervision.
Ability to perform a variety of routine procedures.
Ability to prioritize work.
Brigham And Women's Hospital