Technical Research Assistant II, 40Hrs, Days, Rheumatology

Wentworth-Douglass Hospital Boston , MA 02298

Posted 4 months ago

GENERAL SUMMARY/ OVERVIEW STATEMENT: Summarize the nature and level of work performed.

This position offers the opportunity to join a group studying the autoimmune diseases lupus and rheumatoid arthritis. Our research group studies the functions of immune cells isolated from patients with these diseases. Recently we discovered a unique T cell population called 'peripheral helper T cells' that is dramatically expanded in the joints of rheumatoid arthritis patients, published in the journal Nature. Our current projects study this newly described T cell population in the hope that these cells can be targeted to treat autoimmune diseases. Under general supervision by a supervisor, the self-motivated individual will perform activities related to the processing and analysis of human and mouse cell and tissue samples using standard immunology techniques, including cell isolation and cell culture, staining cells for flow cytometry, performing ELISAs and RT-PCR. Responsibilities also include tracking and organizing tissue and cell samples, developing analysis methods, maintaining regulatory protocols, data analysis, and preparation of data presentations. In addition, this individual will perform a variety of routine assays and experiments in the laboratory and help in general to maintain lab organization and support laboratory staff.

PRINCIPAL DUTIES AND RESPONSIBILITIES: Indicate key areas of responsibility, major job duties, special projects and key objectives for this position. These items should be evaluated throughout the year and included in the written annual evaluation.
1.Process samples for research, including:

  • Processing of tissue samples and isolation of cells

  • Ficoll purification of mononuclear cells

  • Isolation and culture of T cells or other cell types from tissue specimens

  • Cell staining for flow cytometry, cell sorting, and mass cytometry

  • Isolation and analysis of RNA

  • Aliquot and freeze or thaw biospecimens

  • Perform T cell experiments, including:

  • Cell stimulation for detection of cytokines by RTPCR, ELISA, flow cytometry

  • Functional assays

  • Co-culture of T cells with other cell types

  • Work independently under general supervision, perform a variety of routine assays and experiments in the laboratory including (but not limited to):

  • Short-term and long-term cell cultures requiring aseptic cell culture technique

  • Functional in vitro cell culture assays

  • ELISA

  • Flow cytometry

  • PCR/qPCR
    4.Report on progress of work at laboratory seminars.
    5.Prepare laboratory reagents, chemicals, instruments and equipment.
    6.Maintain supplies inventory in accordance with laboratory and department procedures.
    7.Assist in the orientation and training of new students, technicians, and fellows.
    8.Maintain established department policies, procedures and objectives, including the areas of quality assurance, safety, environmental and infection control.
    9.Perform other duties as directed.

QUALIFICATIONS: (MUST be realistic, neither overstated nor understated, and related to the essential functions of the job.)

Bachelor's degree or equivalent required.

At least one year experience in a biomedical research lab.

Demonstrated organizational and interpersonal skills as required to work closely with a research team.

Demonstrated competence with databases/spreadsheets and software packages.

Willingness to learn other laboratory techniques.

SKILLS/ ABILITIES/ COMPETENCIES REQUIRED: (MUST be realistic, neither overstated nor understated, and related to the essential functions of the job.)

Must possess good organizational skills.

Ability to communicate information clearly in both oral and written form, including email.

Ability to make decisions based on logical assumptions of factual data to solve technical and logistical problems.


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Technical Research Assistant II, 40Hrs, Days, Rheumatology

Wentworth-Douglass Hospital