Technical Research Assistant II/ 40/ Day/ Bwh/ Pathology

Brigham And Women's Hospital Boston , MA 02298

Posted 3 months ago

GENERAL SUMMARY:

Under very general supervision of Principal Investigator, and in accordance with established hospital policies and procedures, conducts experiments to identify new therapeutic strategies in pediatric and adult cancers (previous studies by the lab have led to FDA approval of many new drugs for cancer). This research assistant position is cell culture intensive and uses viability, protein (immunoblotting), and flow cytometry analytic techniques to evaluate tumor cell cultures after treatment with novel therapies. The position also includes responsibility (along with other lab members) for genome sequencing and other high-throughput analytics to identify therapeutically-relevant biologic vulnerabilities in cancer.

These studies are coupled with functional validations (gene knockdowns and overexpression) to confirm and understand the therapeutic opportunities. Ideally, the research assistant will look forward to preparing manuscripts for publication in high-impact science and medicine journals, so strong writing ability is a plus. The research assistant will also assist in the training of new students and postdoctoral fellows in standard methods of molecular biology and cell culture. Perfect opportunity for someone planning to attend medical or graduate school in 2-3 years!

This laboratory is primarily interested in the biology and therapeutics of cancer.

PRINCIPAL DUTIES AND RESPONSIBILITIES:
1.Independently performs routine and non-routine experiments which are moderately complex. Carries out methods of molecular biology including: Establishing and maintaining cell cultures from cancer biopsy specimens; Evaluating cancer cell culture responses to drug treatments, using cell proliferation, apoptosis, flow cytometry, and immunoblotting assays; Isolation of various cell fractions from blood, bone marrow and solid tumor specimens; Polymerase chain reaction DNA amplification; Immunoprecipitation and immunoblotting analyses of protein function; DNA cloning in phage and plasmids; DNA sequencing; fluorescence in situ hybridization.
2.Develops and implements new molecular biology protocols aimed at improving efficiency of DNA library cloning and sequencing, DNA transfections and transductions, and cell response assays for viability, proliferation, migration and invasion.
3.Calculates, transcibes and analyzes data using advanced statistical techniques.
4.Prepares and presents oral and written progress reports on experiments to Principal Investigator.
5.Reports on progress of work at weekly laboratory meetings. Also, assists in writing up experimental work for grant applications, book chapters and journal publications.
6.Trains new students, residents, postdoctoral fellows and collaborators in the developmental protocols described above.
7.Prepares laboratory reagents, chemicals, instruments and equipment.
8.Maintains supplies inventory in accordance with department procedures.
9.Maintains established department policies, procedures and objectives, including the areas of quality assurance, safety, environmental and infection control.
10. Performs all other duties and responsibilities as directed.

QUALIFICATIONS:

Bachelor of Science or Bachelor of Arts degree in a biological/physical science required.

Must have at least one year experience in a directly related research setting.

Undergraduate experience with Creative Writing (or other courses that focus on writing skills, such as critical or analytical writing) is a plus.

SKILLS/ABILITIES/COMPETENCIES REQUIRED:

Requires excellent analytical skills.

Must be able to logically and effectively structure tasks and set priorities.

Ability to perform a variety of highly specialized molecular biology procedures.

Must possess strong oral and written communication skills.

On own initiative, ability to identify potential problems and trouble-shoot problems as necessary.

Ability to work independently.



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VIEW JOBS 11/9/2019 12:00:00 AM 2020-02-07T00:00 GENERAL SUMMARY: Under very general supervision of Principal Investigator, and in accordance with established hospital policies and procedures, conducts experiments to identify new therapeutic strategies in pediatric and adult cancers (previous studies by the lab have led to FDA approval of many new drugs for cancer). This research assistant position is cell culture intensive and uses viability, protein (immunoblotting), and flow cytometry analytic techniques to evaluate tumor cell cultures after treatment with novel therapies. The position also includes responsibility (along with other lab members) for genome sequencing and other high-throughput analytics to identify therapeutically-relevant biologic vulnerabilities in cancer. These studies are coupled with functional validations (gene knockdowns and overexpression) to confirm and understand the therapeutic opportunities. Ideally, the research assistant will look forward to preparing manuscripts for publication in high-impact science and medicine journals, so strong writing ability is a plus. The research assistant will also assist in the training of new students and postdoctoral fellows in standard methods of molecular biology and cell culture. Perfect opportunity for someone planning to attend medical or graduate school in 2-3 years! This laboratory is primarily interested in the biology and therapeutics of cancer. PRINCIPAL DUTIES AND RESPONSIBILITIES: 1. Independently performs routine and non-routine experiments which are moderately complex. Carries out methods of molecular biology including: Establishing and maintaining cell cultures from cancer biopsy specimens; Evaluating cancer cell culture responses to drug treatments, using cell proliferation, apoptosis, flow cytometry, and immunoblotting assays; Isolation of various cell fractions from blood, bone marrow and solid tumor specimens; Polymerase chain reaction DNA amplification; Immunoprecipitation and immunoblotting analyses of protein function; DNA cloning in phage and plasmids; DNA sequencing; fluorescence in situ hybridization. 2. Develops and implements new molecular biology protocols aimed at improving efficiency of DNA library cloning and sequencing, DNA transfections and transductions, and cell response assays for viability, proliferation, migration and invasion. 3. Calculates, transcibes and analyzes data using advanced statistical techniques. 4. Prepares and presents oral and written progress reports on experiments to Principal Investigator. 5. Reports on progress of work at weekly laboratory meetings. Also, assists in writing up experimental work for grant applications, book chapters and journal publications. 6. Trains new students, residents, postdoctoral fellows and collaborators in the developmental protocols described above. 7. Prepares laboratory reagents, chemicals, instruments and equipment. 8. Maintains supplies inventory in accordance with department procedures. 9. Maintains established department policies, procedures and objectives, including the areas of quality assurance, safety, environmental and infection control. 10. Performs all other duties and responsibilities as directed. QUALIFICATIONS: Bachelor of Science or Bachelor of Arts degree in a biological/physical science required. Must have at least one year experience in a directly related research setting. Undergraduate experience with Creative Writing (or other courses that focus on writing skills, such as critical or analytical writing) is a plus. SKILLS/ABILITIES/COMPETENCIES REQUIRED: Requires excellent analytical skills. Must be able to logically and effectively structure tasks and set priorities. Ability to perform a variety of highly specialized molecular biology procedures. Must possess strong oral and written communication skills. On own initiative, ability to identify potential problems and trouble-shoot problems as necessary. Ability to work independently. Partners Healthcare System Boston MA

Technical Research Assistant II/ 40/ Day/ Bwh/ Pathology

Brigham And Women's Hospital