Working independently, under general supervision of the PhD investigators in the lab, executes protocols involving a variety of routine and non-routine experiments. May identify new techniques. Develops modification of existing techniques, as appropriate. In collaboration with other members of the research team, gives input to the development of design protocols.
PRINCIPAL DUTIES AND RESPONSIBILITES:
Establish primary cell lines from fresh tissue (cryopreserve and store)
Perform routine tissue culture maintenance of cell lines
Extract and QC nucleic acid (DNA and RNA) from blood and frozen tissue.
Isolate protein from fresh tissue
Collect PBMCs, serum, pleural effusion
Perform PK, PRP, and other blood work
Organize, store and keep inventory of FFPE Blocks, frozen samples, cell lines
Support the clinical research associates on various clinical trials
Prepare of reagents and media used in lab experiments
Assist the supervisor in research projects
Responsible for accurate documentation and data recording of all samples
Discuss progress of work regularly with the supervisor.
All other duties, as assigned.
Maintain laboratory supplies; keep inventory, and order items for the laboratory
Autoclave tubes, flasks, probes and other items
Properly dispose of hazardous material (fill out tags and online form)
Monitor LN2 and CO2 levels
Wash and autoclave laboratory glassware
Assist in orientation and training of interns and new staff
BA/BS in biology or a related field required
Experience with tissue culture and acid nucleic extraction required
Strong preference will be given to individuals that are willing to spend more than 3 years as a technician in the laboratory.
Good organizational skills, detail oriented
Sound degree of computer literacy
Ability to methodically record data
Ability to perform a variety of routine tests and prioritize work
Good interpersonal and communication skills
Ability to demonstrate professionalism