Technical Research Assistant I * Thoracic Surgery / 40 Hours / Day / BWH Dept. Of Surgery

Wentworth-Douglass Hospital Boston , MA 02298

Posted 6 months ago


Working independently, under general supervision of the PhD investigators in the lab, executes protocols involving a variety of routine and non-routine experiments. May identify new techniques. Develops modification of existing techniques, as appropriate. In collaboration with other members of the research team, gives input to the development of design protocols.


  • Establish primary cell lines from fresh tissue (cryopreserve and store)

  • Perform routine tissue culture maintenance of cell lines

  • Extract and QC nucleic acid (DNA and RNA) from blood and frozen tissue.

  • Isolate protein from fresh tissue

  • Collect PBMCs, serum, pleural effusion

  • Perform PK, PRP, and other blood work

  • Organize, store and keep inventory of FFPE Blocks, frozen samples, cell lines

  • Support the clinical research associates on various clinical trials

  • Prepare of reagents and media used in lab experiments

  • Assist the supervisor in research projects

  • Responsible for accurate documentation and data recording of all samples

  • Discuss progress of work regularly with the supervisor.

  • All other duties, as assigned.

Additional responsibilities:

  • Maintain laboratory supplies; keep inventory, and order items for the laboratory

  • Autoclave tubes, flasks, probes and other items

  • Properly dispose of hazardous material (fill out tags and online form)

  • Monitor LN2 and CO2 levels

  • Wash and autoclave laboratory glassware

  • Assist in orientation and training of interns and new staff


  • BA/BS in biology or a related field required

  • Experience with tissue culture and acid nucleic extraction required

  • Strong preference will be given to individuals that are willing to spend more than 3 years as a technician in the laboratory.


  • Good organizational skills, detail oriented

  • Sound degree of computer literacy

  • Ability to methodically record data

  • Ability to perform a variety of routine tests and prioritize work

  • Good interpersonal and communication skills

  • Ability to demonstrate professionalism

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Technical Research Assistant I * Thoracic Surgery / 40 Hours / Day / BWH Dept. Of Surgery

Wentworth-Douglass Hospital