Technical Research Assistant I * Thoracic Surgery / 40 Hours / Day / BWH Dept. Of Surgery

Wentworth-Douglass Hospital Boston , MA 02298

Posted 6 months ago

GENERAL SUMMARY:

Working independently, under general supervision of the PhD investigators in the lab, executes protocols involving a variety of routine and non-routine experiments. May identify new techniques. Develops modification of existing techniques, as appropriate. In collaboration with other members of the research team, gives input to the development of design protocols.

PRINCIPAL DUTIES AND RESPONSIBILITES:

  • Establish primary cell lines from fresh tissue (cryopreserve and store)

  • Perform routine tissue culture maintenance of cell lines

  • Extract and QC nucleic acid (DNA and RNA) from blood and frozen tissue.

  • Isolate protein from fresh tissue

  • Collect PBMCs, serum, pleural effusion

  • Perform PK, PRP, and other blood work

  • Organize, store and keep inventory of FFPE Blocks, frozen samples, cell lines

  • Support the clinical research associates on various clinical trials

  • Prepare of reagents and media used in lab experiments

  • Assist the supervisor in research projects

  • Responsible for accurate documentation and data recording of all samples

  • Discuss progress of work regularly with the supervisor.

  • All other duties, as assigned.

Additional responsibilities:

  • Maintain laboratory supplies; keep inventory, and order items for the laboratory

  • Autoclave tubes, flasks, probes and other items

  • Properly dispose of hazardous material (fill out tags and online form)

  • Monitor LN2 and CO2 levels

  • Wash and autoclave laboratory glassware

  • Assist in orientation and training of interns and new staff

QUALIFICATIONS:

  • BA/BS in biology or a related field required

  • Experience with tissue culture and acid nucleic extraction required

  • Strong preference will be given to individuals that are willing to spend more than 3 years as a technician in the laboratory.

SKILLS/ABILITIES/COMPETENCIES REQUIRED:

  • Good organizational skills, detail oriented

  • Sound degree of computer literacy

  • Ability to methodically record data

  • Ability to perform a variety of routine tests and prioritize work

  • Good interpersonal and communication skills

  • Ability to demonstrate professionalism

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Technical Research Assistant I * Thoracic Surgery / 40 Hours / Day / BWH Dept. Of Surgery

Wentworth-Douglass Hospital