The Weiner lab and the CLIMB Study team at the Ann Romney Center for Neurologic Diseases is seeking to hire a highly qualified and motivated Technical Research Assistant I to work with our microbiome center and our multiple sclerosis biorepository. Work will be focused on sample processing (blood and stool) and maintenance of the biorepository (sample logging and retrieval, maintaining and auditing sample inventory, and performing quality control assessments). In addition, the incumbent will assist with experimental work aimed at manipulating the microbiome in animal models of neurologic disease and profiling the microbiome with high-throughput sequencing.
Working under the general supervision of Principal Investigator, and in accordance with established hospital policies and procedures, conducts a variety of routine and non-routine experiments where methodologies are clearly established or being developed together with Principal Investigator.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Under general supervision, performs a variety of routine and non-routine tests following established methodologies.
Processing and freezing biologic specimens (blood and stool) from patients collected as part of an ongoing longitudinal study of patients with multiple sclerosis. Aliquoting and freezing of stool samples.
Maintaining a repository of frozen samples for future and ongoing studies. Recording samples in an Oracle based LIMS system. Troubleshooting logging errors and performing audits of sample inventory. Performing quality control assessments of frozen samples.
Performing routine molecular experiments, including DNA extraction, and 16S rRNA amplicon library preparation under supervision.
Assisting with experimental work including, but not limited to, animal care, assays such as flow cytometry, flow-assisted cell-sorting (FACS), DNA and RNA isolation, transcriptional profiling (real-time rt-PCR, RNA sequencing, nanostring), proliferation assays, ELISA, and Luminex. Performs data analysis and preliminary statistical analysis under supervision.
Recruitment of healthy volunteers to donate blood as controls; obtaining informed consent.
Involved in orientation and training of other employees, such as new technicians and summer interns. Assists in the day-to-day supervision of high school interns.
Discusses progress of work regularly with supervisor.
Maintains laboratory notebook of daily activities including all experiments and procedures.
Conducts literature searches and review, compiling information from journals and suggesting uses of new techniques of research and laboratory techniques, consulting with other researchers and techniques to exchange information.
Attends courses in safety procedures for the handling, storage and disposal of biohazardous, infectious, radioactive and toxic substances, ensuring that all laboratory activities are carried out in compliance with such procedures.
Performs other duties as assigned or as position requires.
BS in a biological/physical science with hands-on experience in any biology field required. Should have previous experience of working in a research lab and familiarity with basic laboratory instruments, equipment, and scientific procedures.
May consider a bachelor's degree in another area of concentration, if individual has had some course work in the sciences and possesses practical experience in basic laboratory techniques.
Must have a sound understanding of laboratory practices and procedures.
Ability to manage work processes in a neat and orderly way, to follow instructions, and to work both independently and collaboratively as a member of a team.
Ability to multi-task and prioritize work with attention to detail and punctuality.
On own initiative, ability to identify potential problems and troubleshoot problems as necessary.
Must have good manual dexterity and be able to carry items weighing up to 30 lbs.
Good organizational and record keeping skills, detail oriented.
Good interpersonal and communication skills.
Ability to demonstrate professionalism.
Sound degree of computer literacy.
Normal research laboratory environment. May be exposed to radioactive and other biohazardous materials including human blood products, under controlled conditions. Minimal amount of heavy lifting or bending involving moving of laboratory equipment and supplies.
Partners Healthcare System