Technical Research Assistant I * Burn /Trauma / 40 Hours / Day / BWH Surgery

Wentworth-Douglass Hospital Boston , MA 02298

Posted 6 months ago

GENERAL SUMMARY/ OVERVIEW STATEMENT: Summarize the nature and level of work performed.

This position is for a technician to perform basic cellular immunology research using mass cytometry (CyTOF) to characterize and phenotype human and mouse immune cell subsets. The technician will work on studies to profile human blood immune cells from patients with rheumatoid arthritis (RA) or systemic lupus erythematosus (SLE) diseases or trauma patients.

Mouse disease model studies will also be used to study immune cell responses to injury, infections, and radiation. The technician will be an integral part of these ongoing human and mouse research studies. The day-to-day activities will involve validating and testing CyTOF antibody panels, cell preparation, cell staining with CyTOF antibodies, and data analysis. The technician will also be involved with ordering supplies, preparing specialized reagents, and helping others in the lab with CyTOF technology.

PRINCIPAL DUTIES AND RESPONSIBILITIES: Indicate key areas of responsibility, major job duties, special projects and key objectives for this position. These items should be evaluated throughout the year and included in the written annual evaluation.
1.Help with organization of the Harvard Medical Area (HMA) CyTOF antibody resource and core.
2.Prepare human and mouse blood and tissue cells for experiments and to validate CyTOF-ready antibodies
3.Consult and interact with CyTOF users on the technical features of the technology
5.Analyze CyTOF staining data using online software for multidimensional cell stain data
6.Train others in the laboratory on using CyTOF technology
7.Work as a team on specific studies to profile human and mouse blood cells or tissue cells to identify disease-specific phenotypes.

QUALIFICATIONS: (MUST be realistic, neither overstated nor understated, and related to the essential functions of the job.)
1.BS, BA, or MS degree majoring in biology, microbiology, chemistry, or related biological sciences.
2.Especially interested in individuals that wish to apply to medical or graduate school in the future.

SKILLS/ ABILITIES/ COMPETENCIES REQUIRED: (MUST be realistic, neither overstated nor understated, and related to the essential functions of the job.)
1.Basic laboratory skills
2.Computational skills and knowledge of how to use database programs
3.Organizational skills and ability to focus on details
4.Good interpersonal and communication skills
5.Ability to demonstrate professionalism

QUALIFICATIONS: (MUST be realistic, neither overstated nor understated, and related to the essential functions of the job.)
1.BS, BA, or MS degree majoring in biology, microbiology, chemistry, or related biological sciences.
2.Especially interested in individuals that wish to apply to medical or graduate school in the future.



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Technical Research Assistant I * Burn /Trauma / 40 Hours / Day / BWH Surgery

Wentworth-Douglass Hospital