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SRA I- Kuroda Lab

Expired Job

University Of California Davis , CA 95616

Posted 3 months ago

Under supervision of the lab manager, provide ongoing technical, scientific, and organizational support in the operation of the laboratory research activities focused on research of the role of monocyte/macrophages in infectious disease pathogeneses and on the immunology of aging. Provide support in tissue culture and isolation/propagation of infectious agents and process tissue samples of nonhuman primates for studies of viral infection.

Assist in the implementation of research experiments, and perform both simple and complex assays, including immunological and enzyme-based assays. Assist in molecular biology techniques, such as flow cytometry, Immunocytochemistry, RNA/DNA isolation, and colorimetric assays. Assist in data analysis.

Work according to Standard Operating Procedures (SOP) including but not limited to: maintenance of laboratory reagents and equipment, inventory of laboratory supplies, and maintenance of biospecimen inventories. Provide general laboratory maintenance.

Physical Demands:

Lift, move and/or maneuver (with equipment) items weighing up to 50 lbs.

Stand or sit in one place for extended periods of time.

Use microscopes on a frequent basis.

Perform procedures for cryopreservation that include liquid nitrogen handling.

Must be willing to perform assigned duties in BioSafety Level (BSL) 2/3 condition with specimens from non-human primates that may be naturally or experimentally infected with potentially hazardous infectious agents such as SRV, SIV, HIV, STLV, HTLV, and herpesvirus.

Must be willing to perform assigned duties in areas where hazardous materials are present.

Must be willing to perform assigned duties using the following equipment: biological safety cabinets, chemical fume hoods, pH meter, rotors and centrifuges, laboratory instruments such as sonicators and gel electrophoresis, spectrophotometers, and flow cytometers.

Must be willing to comply with safety precautions within the laboratory and attend all safety meetings, as requested.

Work Environment:

This position is subject to Medical Surveillance procedures, which include requirement of measles vaccination (or documentation of illness) and TB clearance, and review in accordance with Federal and State Laws and Regulations and University Policy.

Work with recombinant viral vectors and with infectious viruses and bacteria, and processing specimens from human and nonhuman primates routinely and handling chemical agents.

Position requires employees to work with or be in areas where hazardous materials and/or infectious agents are present.

Employee is personally responsible for following health and safety guidelines/instruction, including wearing appropriate personal protective equipment.

Work occasional overtime including some weekends and holidays, and ability to travel to other UC Davis campus locations to use specialized research equipment.

UC Davis is a smoke and tobacco free campus effective January 1, 2014. Smoking, the use of smokeless tobacco products, and the use of unregulated nicotine products (e-cigarettes) will be strictly prohibited on any UC Davis owned or leased property, indoors and outdoors, including parking lots and residential space.

Background Check Required:

This position is a critical position and subject to a background check. Employment is contingent upon successful completion of background investigation including criminal history and identity checks.

Yes

Qualifications

Minimum Qualifications:

Academic background in Molecular Virology, Biochemistry, Molecular Biology, Cellular Biology, Immunology, or related field(s).

Experience in concepts, principles and conduct of biomedical research.

Experience in basic tissue culture and virus work, including cryopreservation in liquid nitrogen.

Experience in aseptic technique.

Experience in isolating and culturing primary cells.

Experience in laboratory research on infectious agents.

Experience in basic immunology and enzyme-based experimental principles and skill to perform assays.

Experience in operating various lab equipment, including use of low and high speed centrifuges; pH meters; water purification systems; biosafety cabinets; chemical fume hoods; microscopes, CO2 incubators, autoclaves, and lab balances.

Experience in operating plate washers and spectrophotometers.

Experience in performing mathematical calculations for concentrations and volumes of solutions.

Experience in the safe use and handling/disposal of biohazardous/chemical waste.

Experience in preparing and sterilizing lab reagents, cell culture media, and buffers.

Experience in record keeping skills and electronic data management.

Experience in scientifically describing in detail the experiments performed and recording descriptions in notebooks (electronic and handwritten).

Experience in maintaining organized lab records including purchasing records, chemical inventories, freezer logs, and preparation of media and various lab reagents.

Experience in computer database and word-processing skills.

Experience in housekeeping activities including stocking general laboratory supplies, washing glassware/plasticware used for making/storing lab solutions.

Familiarity in quality control of immunological data.

Familiarity in the basic principles of molecular biology, including isolation and purification of DNA/RNA and protein purification.

Familiarity in the principles of flow cytometry and confocal microscopy.

Familiarity in the principles of confocal microscopy.

Familiarity in the basic principles of necropsies and the process of isolating whole tissue samples.

Preferred Qualifications:

Skills in coordinating research activities in different projects so as to manage time efficiently (i.e. multi-tasking).

Skills with computer applications for transcribing raw data and analyzing results, including Word, Excel, and Powerpoint software.

Skills in using chemical and infectious agent precautions and ability to follow and uphold safety protocols.

Skills in using biosafety cabinets, PCR machines, microscope, centrifuges, sterile filtration systems, balances, autoclaves, and pH meters.

Skills to mathematically calculate concentrations, molarity, and volumes for lab reagents.

Skills in following lab protocols or SOP.

Skills in aseptic techniques.

Skills to perform virology and immunological procedures, including but not limited to, virus growth and titration, RNA/DNA isolation.



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SRA I- Kuroda Lab

Expired Job

University Of California