As a member of the Vector Core group this molecular biologist position will help drive the development of Sarepta's Gene Therapy programs and be responsible for the design and characterization of plasmid constructs for early-stage production processes for AAV vector research pipeline. The team will collaborate with Analytical Development, Process Development and additional internal and external stakeholders to ensure the development and transfer of robust processes to facilitate fit for purpose clinical and commercial AAV vector production.
Primary Responsibilities include:
Provide leadership role in building and optimizing the design of AAV gene therapy constructs
Responsible for hands-on experimental design, execution, data analysis and reports for AAV vector core pipeline research and development programs
Develop molecular assays to characterize AAV genome integrity, identity, vector variants, residual DNA quantification, as well as in-vitro potency to support product development and manufacturing of gene therapy products
Identification and implementation of operational improvements and novel technologies to improve AAV vector expression and key quality attributes
Participate in project related teams and other internal/external collaborations
Prepare and review technical reports and SOPs to support CMC regulatory activities
In collaboration with Gene Therapy Technical Operations Leadership, develop strategic and operational vision for the organization
Select, develop, and evaluate personnel to ensure the efficient operation of the function and represent the department internally/externally
Work seamlessly with cross functional stakeholders including Process Development, Analytical Development and Translational Research to help advance the AAV vector production portfolio
Ensure the development of robust, well-characterized, high quality and high yielding vector expression system
Ensure that scientific and technical challenges are resolved in a timely manner
Contribute to budget and goal planning
Contribute to a safe, efficient and effective environment with personal accountability
Education and Skills Requirements:
Ph.D. with 6+ years related experience or MS with 10+ years related experience with degree in Molecular/Cellular Biology, Virology or a related discipline
Deep understanding of plasmid expression cassettes, vector biology and molecular techniques
Extensive hands-on experience with molecular and cellular assays critical for biologics drug discovery or gene therapy development research including plasmid cloning, E. coli screening, restriction digestion mapping, DNA sequencing, qPCR, RT-qPCR, gel or capillary electrophoresis, and in vitro plasmid expression
Experience in ELISA and western blot is required
High-throughput qPCR experience is preferred
Experience in ddPCR and NGS is desirable
Experience in process design utilizing QbD and DOE principles via high-throughput small-scale approaches for optimization of viral vectors or biologics production is required
Able to gather internal/external benchmarks for best practices in the scientific field
Familiar with upstream techniques used in the growth of biologics including suspension and adherent culture of mammalian or insect cells
Solid communication and interpersonal skills to help build strong relationships within and across various drug discovery and development functions
Candidates must be authorized to work in the U.S.
Sarepta Therapeutics offers a competitive compensation and benefit package.
Sarepta Therapeutics is an Equal Opportunity/Affirmative Action employer and participates in e-Verify.