Senior Scientist, Antibody Discovery & Engineering, Single B Cell Cloning

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At Takeda, we are a forward-looking, world-class R&D organization that unlocks innovation and delivers transformative therapies to patients. By focusing R&D efforts on four therapeutic areas and other targeted investments, we push the boundaries of what is possible in order to bring life-changing therapies to patients worldwide. Join Takeda as a Senior Scientist where you will have industrial experience in state-of-the-art antibody-based discovery using in vivo immunization strategies, repertoire analysis using both NGS and Sanger sequencing methods, high throughput single B cell cloning, engineering including affinity maturation/ stability engineering/humanization.

You will necessitate familiarity with associated screening workflows and data analysis in addition to possessing a deep knowledge of B cell biology, humoral immunity and immunoglobulin genetics and familiarity with basic building block biologics IgG, Fab, sdAb (knowledge of bispecifics is nice to have). Designing and optimizing lead candidates using display-based technologies (yeast, phage) may also be part of the role. You will also recognize, and streamline for the most efficient ways of working is a high priority in this role, and have tremendous drive, motivation to deliver (and beat), team and program goals, while also pushing the boundaries to enhance discovery and optimization. Working / networking excellently within the team is critical for best performance and team engagement.

As part of the Biotherapeutic Engineering team, you will report to the Senior Director and be part of a team of motivated and enthusiastic scientists in the Cambridge MA location, bringing strong experience in antibody/biologic discovery to the team. How you will contribute: Thoughtfully design and implement, through hands-on work, successful in vivo antibody discovery campaigns in human transgenic mice using a versed array of approaches (DNA/RNA/protein and cell-based immunization strategies). Oversee same for llamas.

Mine repertoires using B cell cloning approaches (FACS and Berkeley Lights Beacon-based technologies) for VHH and IgG-based workflows including sorting, cloning and sequencing protocols to enable fast and efficient lead discovery. Use NGS to complement in vivo-based discovery and repertoire expansion. Perform sequence analysis through state-of-the-art tools for deep understanding and performance of campaigns.

Work with data analysis tools to ensure thorough evaluation of datasets and information based next step decision-making, in addition to summarize and present findings to smaller and larger teams. Work with wider team to design maturation libraries (affinity/ potency/ stability) using experience and software to improve lead candidates using display-based technologies. High level of diligence in all data tracking, uploading, and recording using data management systems within the department.

Be an effective team member of discovery programs, collaborate and interact with team members and other internal groups for high efficiency and transparency to enable progression of molecules. Demonstrate pro-activity through identifying areas to improve efficiency, perform technology development to bring innovation to the team, where appropriate. Independently design, develop and execute research assignments and contribute to multiple projects, in parallel.

Work efficiently with a sense of urgency to meet program and team needs. Contribution to the preparation of patents, documentation for IND, and publications. Minimum Requirements/Qualifications:

PhD degree in a scientific discipline (or equivalent) with 2 years relevant experience, or MS with 8 years relevant experience, or BS with 10 years relevant experience. Excellent experience of in vivo immunization strategies and handling antibody discovery platforms, specifically single B cell cloning, with adeptness in FACS based sorting strategies. Excellent knowledge of antibody discovery and engineering, B cell biology, humoral immunity and immunoglobulin genetics.

Good familiarity with antibody repertoire analysis- NGS data handling and analysis from either in vivo or in vitro sources. More about us: At Takeda, we are transforming patient care through the development of novel specialty pharmaceuticals and best in class patient support programs.

Takeda is a patient-focused company that will inspire and empower you to grow through life-changing work. Certified as a Global Top Employer, Takeda offers stimulating careers, encourages innovation, and strives for excellence in everything we do. We foster an inclusive, collaborative workplace, in which our teams are united by an unwavering commitment to deliver Better Health and a Brighter Future to people around the world.

LI-SGM LI-Onsite Takeda Compensation and Benefits Summary We understand compensation is an important factor as you consider the next step in your career. We are committed to equitable pay for all employees, and we strive to be more transparent with our pay practices. For Location:

Boston, MA U.S. Base Salary Range: $133,000.00 - $209,000.00 The estimated salary range reflects an anticipated range for this position. The actual base salary offered may depend on a variety of factors, including the qualifications of the individual applicant for the position, years of relevant experience, specific and unique skills, level of education attained, certifications or other professional licenses held, and the location in which the applicant lives and/or from which they will be performing the job.The actual base salary offered will be in accordance with state or local minimum wage requirements for the job location.

U.S. based employees may be eligible for short-term and/or long-termincentives. U.S.based employees may be eligible to participate in medical, dental, vision insurance, a 401(k) plan and company match, short-term and long-term disability coverage, basic life insurance, a tuition reimbursement program, paid volunteer time off, company holidays, and well-being benefits, among others. U.S.based employees are also eligible to receive, per calendar year, up to 80 hours of sick time, and new hires are eligible to accrue up to 120 hours of paid vacation.

EEO Statement Takeda is proud in its commitment to creating a diverse workforce and providing equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, gender expression, parental status, national origin, age, disability, citizenship status, genetic information or characteristics, marital status, status as a Vietnam era veteran, special disabled veteran, or other protected veteran in accordance with applicable federal, state and local laws, and any other characteristic protected by law. Locations Boston, MA Worker Type Employee Worker Sub-Type Regular Time Type Full time