Senior Research Technician - Experimental Medicine

Dana-Farber Cancer Institute Brookline , MA 02445

Posted 2 weeks ago

Overview

Exciting opportunity for an experienced, motivated research technician to join a translational laboratory studying various aspects of cellular adaptive immunity, especially in relation to T regulatory cell characterization, function and expansion, as well as the effects of various immune manipulations. We also have separate, secondary focus on innate immunity, particularly in regard to effects on hematopoiesis and chemoradiotherapy toxicity. We have a history of successfully conducting basic and preclinical experiments on new biologic agents and translating them into the clinic, and currently collaborate in clinical trials studying the adaptive use of T regulatory cells in transplant settings.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

Responsibilities

  • A successful candidate will have the following characteristics:

  • Reliable, organized, and flexible with ability to handle multiple activities and to reprioritize tasks

  • Understands lab etiquette and participation in general laboratory responsibilities (ordering and organizing laboratory supplies and reagents)

  • Able to work with other team members and collaborators as well as complete independent work

  • Able to work closely with supervisor and staff scientists to analyze data and interpret results of experiments within the context of research project goals, and participate in or take responsibility for SOP generation

  • Experience in analyzing biological data using GraphPad Prism and Excel required. Experience with FlowJo and SoftMax Pro and any array analysis experience strong positives.

Qualifications

  • Relevant experience in academia or industry strongly preferred. Candidate should have Master's Degree although Bachelor's degree with adequate experience considered. Coursework in related fields (such as Immunology and Molecular Biology) a plus.

  • Minimum of 2-4 years related experience.

  • Knowledge of information and techniques related to either biology, chemistry, or medicine is preferred.

  • Must have excellent communication skills (written and verbal) and be able to work with a wide variety of faculty and staff.

  • Ability to analyze information and use logic to address work-related issues and problems.

  • Must have attention to detail and be thorough in completing work tasks.

  • Ability to work within a team environment.

Must have human cellular immunology experience, such as isolation of peripheral blood cells for characterization, purification, and cryopreservation; cell culture; sterile technique; cell-based assays including mixed lymphocyte culture and use of various molecular biology and immunology techniques to assess immune responses. Flow cytometry experience highly desirable and cell sorting experience a strong plus. Prior experience with T cell immunology also a strong plus. Other relevant experience includes multianalyte analysis (e.g. Luminex), ELISA, DNA and RNA isolation, PCR and RT-PCR, genechip and microarray, assay development and adaptation. Experience in the proper handling and care of mice, including IP and SC injections, small animal surgical techniques, blood and tissue sample collections required.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.


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