Jounce Therapeutics, Inc. is a clinical stage immunotherapy company dedicated to transforming the treatment of cancer by developing therapies that enable the immune system to attack tumors and provide longlasting benefits to patients. Through the use of its Translational Science Platform, Jounce first focuses on specific cell types within tumors to prioritize targets, and then identifies related biomarkers designed to match the right therapy to the right patient. Jounce's lead product candidate, JTX-2011, is a monoclonal antibody that binds to and activates ICOS and is currently in the Phase 2 portion of the Phase 1/2 ICONIC trial. For more information, please visit www.jouncetx.com.
Role / Responsibilities
The Protein Production group at Jounce Therapeutics is looking for an individual experienced in molecular biology, mammalian cell culture, protein purification, and characterization. The successful candidate will play an important role in the discovery and development of novel biotherapeutics for immuno-oncology as part of a small, highly dedicated team. He/she will be will be responsible for the production of antibodies and proteins for use in R&D that will enable the development of groundbreaking therapies. He/she will be expected to participate in experimental design, conduct experiments, optimize throughput, maintain detailed records, and generate standard operating procedures. This is an exciting opportunity to be part of a fast-paced environment in the dynamic field of immuno-oncology.
Responsibilities may include, but are not limited to:
Transient protein production in mammalian cells
Protein purification steps including affinity chromatography, IEX, SEC, etc.
Protein quality control SE-HPLC, SDS-PAGE, endotoxin assays, etc.
Determination of binding affinities by Octet
Developing methods for automating cell culture transfection, protein purification and screening assays in high throughput fashion using liquid handling robots
Operation of liquid handling robots such as Hamilton Star and Tecan
Thorough documentation and cross-functional communication
BS or M.S. in biochemistry, bioengineering, or a related discipline with 2 + years of biotech/pharma industry experience
Experience with mammalian cell culture, transient and stable mammalian (CHO and HEK) protein expression, and aseptic techniques, required
Experience with automated multi-step protein purification techniques using GE's AKTA systems and Unicorn software, preferred
Experience with analyzing proteins (SE-HPLC, endotoxin assays, SDS-PAGE, Western Blots) required
Experience with Forte Bio Octet, a plus
Experience with high-throughput cell culture expression and protein purification techniques, a plus
Ability to work with LIMS and biological registration systems as well as electronic lab notebook software, required
Excellent attention to detail, strong documentation skills, drive to work in a fast-paced environment, and ability to communicate effectively (both written and verbal), required
Jounce Therapeutics is an equal opportunity employer and does not discriminate in it's employment decisions on the basis of race, color, national origin, age, physical or mental disability, marital status, religion, creed, sex, or political beliefs. We offer a competitive salary and benefits package. All applicants should be legally entitled to work for any employer in the U.S.
To apply, please visit https://careers-jouncetx.icims.com/.