Senior / Principal Scientist - CAR T Cell Construct Engineering - REQ 19024

Lyell Immunopharma, Inc. Seattle , WA 98101

Posted 10 months ago

At Lyell, our vision is to develop curative cell-based immunotherapies for solid tumor cancers. We have innovative science originating from our founders world class labs and a unique and disruptive approach to research and development. Our company is first and foremost focused on understanding the science. We are a learning organization, dependent on deep collaborative relationships between all of our colleagues, partners and founders. Our culture is based on Respect, Science, Courage and Intensity, and it reflects who we are and the environment we are creating.

Position Summary:

We are seeking an independent and self-motivated Senior/Principal Scientist to be a project leader in our CAR T cell construct engineering group. The main goals for this position is to help establish our construct engineering pipeline, integrating streamlined construct generation, testing and validation. This candidate will also help develop ways to utilize gene editing into our pipeline for site directed construct integration. The ideal candidate will integrate new ideas to this pipeline for high throughput cloning, viral and non-viral library generation and automated screening. We would like candidates with previous experience in viral construct engineering, a strong molecular biology background and initiative to dive into a fast-paced new startup and be creative.


Essential Functions:

  • Lead the development of our construct engineering and screening platform for CAR T cell constructs for viral and non-viral delivery
  • Develop ways to automate our cloning and plasmid production for high throughput evaluation of a large range of constructs
  • Manage junior scientists in the lab and aid in their experimental design and execution
  • Willing to wear many hats as is expected in a start up environment
  • Work in a collaborative, multidisciplinary team and help mentor others

Preferred Experience:

  • Extensive experience with both basic and advanced recombinant DNA cloning techniques, including restriction cloning, Golden Gate assembly, Gibson assembly, and PCR-based assembly
  • Demonstrated hands-on experience with molecular techniques, including oligonucleotide and gene fragment design and synthesis, quantification and isolation of DNA and RNA, PCR genotyping, sequencing analysis, media preparation, bacterial culture, sterile technique
  • Extensive knowledge of virus titration methods, copy number determination, integration site analysis and molecular biology techniques such as qPCR and flow cytometry is required
  • Knowledge of genome editing technologies, gRNA design and verification
  • Experience with multi-color flow cytometry and cell sorting for construct testing
  • Familiarity with Geneious, Benchling, or other software for plasmid design and sequence analysis
  • Experience with integrating automation into molecular biology pipeline
  • Great attention to detail, resourceful, and well organized
  • Previous experience managing junior staff
  • Outstanding written, visual, and oral communication skills with a willingness to have ideas challenged and to challenge others ideas in a constructive, efficient and respectful way
  • Start-up /industry experience a plus

Preferred Education:

  • PhD and 8+ years (or equivalent) experience in molecular biology, bioengineering or related discipline with relevant lab comprehension

Preferred Additional


  • Previous experience utilizing viral vectors for gene delivery (preferably in the setting of T cell biology)
  • Preparation and maintenance for process development and research grade plasmid production operations, including SOPs, batch records, reports, and supporting regulatory filings
  • Prior experience working with human samples, specifically primary T cell culture
  • Experience with Electronic Lab Notebook (ELN) systems
  • Demonstrated project management skills


At Lyell, we believe that highest performing teams include people from a wide variety of backgrounds and experiences who respectfully challenge each other. We are committed to building an open, diverse and inclusive culture for all employees.

Weve learned from experience that some of the best people dont always match our requirements perfectly - if youre interested and think you could fit, please dont hesitate to apply.

Lyell is proud to be an equal opportunity employer and does not discriminate on the basis of race, color, citizenship status, national origin, ancestry, sex, sexual orientation, age, religion, creed, physical or mental disability, medical condition, marital status, veteran status or any other characteristics protected under applicable federal, state and local laws.

If you are an individual with a disability and require a reasonable accommodation to complete any part of the application process or are limited or unable to access or use this online application process and need an alternative method for applying, please contact us via our company website.

icon no score

See how you match
to the job

Find your dream job anywhere
with the LiveCareer app.
Mobile App Icon
Download the
LiveCareer app and find
your dream job anywhere
App Store Icon Google Play Icon

Boost your job search productivity with our
free Chrome Extension!

lc_apply_tool GET EXTENSION

Similar Jobs

Want to see jobs matched to your resume? Upload One Now! Remove
Principal Data Scientist

The Climate Corporation

Posted 2 days ago

VIEW JOBS 2/22/2020 12:00:00 AM 2020-05-22T00:00 Position Overview The Climate Corporation is looking for an innovative Principal Data Scientist to lead the development and implementation of core AI solutions to impact the operations and foundations of The Climate Corporation's Science at scale. Working with engineering and scientific leaders, you will set the strategic direction of productizing large scale scientific problems that inform our products and programs. The problems of scaling Agronomic Science via ML and DL are complex and challenging, and you will be leading excellence in Climate's productization strategy. You will need to adopt an architectural vision and work with Machine Learning Engineers and Data Scientists in our team to accelerate the deployment and quality that our growers and enterprise receive. This is a cross-functional role in which you will collaborate with AI Researchers/Engineers as well as Data Engineers. We will remind you that you can do all this while having fun. What You Will Do: * Architect and lead the tactical implementation on the productizing AI frameworks at scale informing Climate and Bayer products and programs * Provide strategic direction to accelerate the deployment of scientific innovation into efficient and robust ML/DL pipelines * Set the standards that take ML/DL capabilities to the next level at Climate and translate them into actionable projects shooting for operationalization excellence * Starting from ambiguous problem definitions, discover new business opportunities and implement optimal and scalable solutions that leverage multiple methodologies, technologies, and skill sets * Mentor and coach a team of talented data scientists and machine learning engineers to adopt an MLOps mentality that empowers the rest of the organization * Build trusted partnerships across the company. Enable functions by supporting them and leading new AI capabilities for the entire company Required Qualifications: * Advanced degree in Statistics, Data Science, Computer Science, Math, Engineering, or other quantitative discipline * 7+ years experience as a technical leader with exceptional hands-on experience in building AI capabilities * 2+ years experience leading the execution of data-intensive projects in a cross-functional setting * Demonstrated experience implementing AI solutions working with a wide-array of data science and engineering infrastructure and relevant cloud solutions * Expert adopter of CD4ML to ensure quality in the ML/DL process * Natural team player and focused on customer needs Preferred Qualifications: * In-depth knowledge of AI technologies and capabilities * Experience translating scientific frameworks into scalable AI solutions * Experience with MLOps best practices, tooling, and infrastructure * Interested in understanding Agronomic Science, Imagery, and Geospatial frameworks to investigate how it can be translated into scalable Data Science to better serve our customers and enterprise What We Offer: Our teams are composed of industry experts, top scientists, and talented engineers. The environment is extremely engaging and fast-paced, with dozens of specialties coming together to provide the best possible products and experiences for our customers. We provide competitive salaries and some of the best perks in the industry, including: * Superb medical, dental, vision, life, disability benefits, and a 401k matching program * A stocked kitchen with a large assortment of snacks & drinks to get you through the day * Encouragement to get out of the office and into the field with agents and farmers to see first-hand how our products are being used * We take part and offer various workshops, conferences, meet-up groups, tech-talks, and hackathons to encourage participation and growth in both community involvement and career development We also hinge our cultural DNA on these five values: * Inspire one another * Innovate in all we do * Leave a mark on the world * Find the possible in the impossible * Be direct and transparent Learn more about our team and our mission: The Climate Corporation - The Technology Behind Making A Difference or visit Climate aims to create a welcoming and collaborative environment for our employees in which a diverse set of perspectives and voices are represented and celebrated. As part of our dedication to the diversity of our workforce, The Climate Corporation is committed to Equal Employment Opportunity and does not discriminate based on race, religion, color, national origin, ethnicity, gender, sex (including pregnancy), protected veteran status, age, disability, sexual orientation, gender identity, gender expression, or any unlawful criterion existing under applicable federal, state, or local laws. If you need assistance or an accommodation due to a disability, you may contact us at The Climate Corporation Seattle WA

Senior / Principal Scientist - CAR T Cell Construct Engineering - REQ 19024

Lyell Immunopharma, Inc.