Senior Associate Scientist - Protein And Antibody Generation And Characterization

Principia Biopharma Inc. Framingham , MA 01702

Posted 1 month ago

Position Overview:

At Sanofi we are committed in our quest to be a world-class biologics research organization. We are pioneering innovation in biologics with the first approved nanobody drug, the first clinical stage tri-specific antibody, and novel synthetic biology for new engineering paths. Our global Large Molecule Research (LMR) platform focuses on antibodies, nanobodies and synthorins as therapeutic modalities to partner closely with other research platforms and Sanofi's therapeutic areas (Immunology, Oncology, Rare Disease and Neurology) in the discovery and ultimate delivery of new candidate molecules into our pipeline.

We are seeking a highly motivated Senior Associate Scientist to join the Biologics Characterization, Expression and Purification group within LMR. The successful candidate will be responsible for the generation and characterization of recombinant proteins to support exciting early discovery and preclinical research projects spanning the therapeutic areas. A range of host cell systems for expression will be used as well as cutting edge automated purification strategies.

Currently the position is located in Framingham MA but will move to Cambridge MA in April 2022.

Key Responsibilities:

  • Expression of recombinant proteins and antibodies employing multiple host cell systems including transient and stable mammalian, microbial and cell free expression.

  • Purification of recombinant proteins and antibodies using affinity-based and/or conventional chromatographic separation techniques; use of automated purification platforms including AKTA.

  • Characterization of produced proteins and antibody drug candidates using SDS-PAGE, analytical SEC, endotoxin analysis, immunoblotting, SPR, flow cytometry, or related techniques.

  • Documentation, analysis and presentation of data in well-organized written and oral formats.

Basic Qualifications:

  • Requires a MS with a minimum of 1 year industry experience, or a BS with a minimum of 4 years industry experience.

  • Past lab experience must include hands on research experience and the ability to apply technical skills in the areas of cell culture, mammalian cell transient transfection and the purification of recombinant proteins.

  • The candidate should have effective communication skills, the ability to work effectively in a collaborative team environment, and present data in well-organized written and oral formats

  • Must have permanent US work authorization

Preferred Qualifications:

  • Experience in protein expression using suspension cultures of mammalian cells (HEK, CHO) as well as insect cells and/or E. coli employing transient and stable expression technologies.

  • Hands on experience using automated protein purification systems such as AKTA or similar.

  • Demonstrated ability to innovate in the areas of cell culture and mammalian cell expression.

  • Prior experience with protein analytical techniques such as SPR, SDS-PAGE, immunoblotting, etc.

  • Prior hands on experience with high throughput expression and purification platforms and/or production and characterization of multi-specific antibody formats.

Sanofi Inc. and its U.S. affiliates are Equal Opportunity and Affirmative Action employers committed to a culturally diverse workforce. All qualified applicants will receive consideration for employment without regard to race; color; creed; religion; national origin; age; ancestry; nationality; marital, domestic partnership or civil union status; sex, gender, gender identity or expression; affectional or sexual orientation; disability; veteran or military status or liability for military status; domestic violence victim status; atypical cellular or blood trait; genetic information (including the refusal to submit to genetic testing) or any other characteristic protected by law.

At Sanofi diversity and inclusion is foundational to how we operate and embedded in our Core Values. We recognize to truly tap into the richness diversity brings we must lead with inclusion and have a workplace where those differences can thrive and be leveraged to empower the lives of our colleagues, patients and customers. We respect and celebrate the diversity of our people, their backgrounds and experiences and provide equal opportunity for all.

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VIEW JOBS 1/6/2022 12:00:00 AM 2022-04-06T00:00 Position Overview: At Sanofi we are committed in our quest to be a world-class biologics research organization. We are pioneering innovation in biologics with the first approved nanobody drug, the first clinical stage tri-specific antibody, and novel synthetic biology for new engineering paths. Our global Large Molecule Research (LMR) platform focuses on antibodies, nanobodies and synthorins as therapeutic modalities to partner closely with other research platforms and Sanofi's therapeutic areas (Immunology, Oncology, Rare Disease and Neurology) in the discovery and ultimate delivery of new candidate molecules into our pipeline. We are seeking a highly motivated Senior Associate Scientist to join the Biologics Characterization, Expression and Purification group within LMR. The successful candidate will be responsible for the generation and characterization of recombinant proteins to support exciting early discovery and preclinical research projects spanning the therapeutic areas. A range of host cell systems for expression will be used as well as cutting edge automated purification strategies. Currently the position is located in Framingham MA but will move to Cambridge MA in April 2022. Key Responsibilities: * Expression of recombinant proteins and antibodies employing multiple host cell systems including transient and stable mammalian, microbial and cell free expression. * Purification of recombinant proteins and antibodies using affinity-based and/or conventional chromatographic separation techniques; use of automated purification platforms including AKTA. * Characterization of produced proteins and antibody drug candidates using SDS-PAGE, analytical SEC, endotoxin analysis, immunoblotting, SPR, flow cytometry, or related techniques. * Documentation, analysis and presentation of data in well-organized written and oral formats. Basic Qualifications: * Requires a MS with a minimum of 1 year industry experience, or a BS with a minimum of 4 years industry experience. * Past lab experience must include hands on research experience and the ability to apply technical skills in the areas of cell culture, mammalian cell transient transfection and the purification of recombinant proteins. * The candidate should have effective communication skills, the ability to work effectively in a collaborative team environment, and present data in well-organized written and oral formats * Must have permanent US work authorization Preferred Qualifications: * Experience in protein expression using suspension cultures of mammalian cells (HEK, CHO) as well as insect cells and/or E. coli employing transient and stable expression technologies. * Hands on experience using automated protein purification systems such as AKTA or similar. * Demonstrated ability to innovate in the areas of cell culture and mammalian cell expression. * Prior experience with protein analytical techniques such as SPR, SDS-PAGE, immunoblotting, etc. * Prior hands on experience with high throughput expression and purification platforms and/or production and characterization of multi-specific antibody formats. Sanofi Inc. and its U.S. affiliates are Equal Opportunity and Affirmative Action employers committed to a culturally diverse workforce. All qualified applicants will receive consideration for employment without regard to race; color; creed; religion; national origin; age; ancestry; nationality; marital, domestic partnership or civil union status; sex, gender, gender identity or expression; affectional or sexual orientation; disability; veteran or military status or liability for military status; domestic violence victim status; atypical cellular or blood trait; genetic information (including the refusal to submit to genetic testing) or any other characteristic protected by law. At Sanofi diversity and inclusion is foundational to how we operate and embedded in our Core Values. We recognize to truly tap into the richness diversity brings we must lead with inclusion and have a workplace where those differences can thrive and be leveraged to empower the lives of our colleagues, patients and customers. We respect and celebrate the diversity of our people, their backgrounds and experiences and provide equal opportunity for all. Apply For Role My Candidate Account Sanofi Framingham MA

Senior Associate Scientist - Protein And Antibody Generation And Characterization

Principia Biopharma Inc.