Pfizer Groton , MA 01450
We are seeking a highly motivated individual who will focus on the generation of cell reagents, to join a team focused on cell provision and cell line development in support of Primary Pharmacology Group and Research Unit needs for in vitro assay development and screening. The successful candidate will support a range of cell reagent deliverables by applying his/her experience with standard mammalian cell culture practices, cell line development, cell banking, and cell line characterization. The candidate will also provide key molecular biology support to enable cell line development and cell characterization, and will help drive continuous improvement in methods and technologies to ensure the highest quality and efficiencies in cell reagent provision.
The successful candidate will join a team of scientists within the Primary Pharmacology Group that is responsible for the delivery of high-quality cell reagents to enable small molecule drug discovery programs. The candidate will support cell culture and provision, often at scale, as well as the development and characterization of stable mammalian cell lines. He/she will execute standard cell culture processes including transfection, selection, sub-cloning, passaging, scale-ups, banking, and characterization and will provide molecular biology support for novel cell line generation and characterization. There may also be opportunities for the candidate to become involved in the production of more complex, disease relevant cellular models from human primary cells or iPSCs.
Successful Candidates will be expected to:
Demonstrate basic laboratory skills to propagate and maintain various continuous and primary cell lines including preparation of medium and mycoplasma testing to ensure cell line sterility.
Be adept at handling human primary cell models and enable the growing application of more physiology relevant models for cellular assays.
Develop stable cell lines by conducting transfections, selection, single cell cloning, screening, characterization, and clone expansion.
Provide molecular biology support for generating and characterizing, and screening cell clones including DNA/RNA extraction, plasmid preparation and cloning, qRT-PCR, and sequence analysis.
Adapt cell culture to large-scale, where applicable, to support mid- to high-throughput screening efforts
Oversee the operation, maintenance, and troubleshooting of key technologies and instruments for cell culture and cell line development.
Help to identify, evaluate, and, where applicable, implement novel methods or technologies that would enhance workflow efficiencies and/or improve the quality of cell reagent deliverables.
Analyze and interpret data, prepare cogent data summaries, and communicate results to partners in the Research Units and Primary Pharmacology Group.
Maintain up-to-date electronic laboratory notebooks and records in accordance with Pfizer policy, and ensure proper archiving of banked cell vials.
Be highly motivated, organized and show significantly ability to work effectively in a team-oriented environment.
BS or MS with 2-5 years relevant laboratory experience required.
Proficient in aseptic mammalian cell culture.
Experienced in the generation, characterization, and expansion of single-cell clones.
Proficient in standard methods for cell characterization including mRNA and protein analysis including qRT-PCR, Western blotting, immunostaining, and FACs.
Hands-on molecular biology experience including nucleic acid/protein isolation, genomic DNA analysis and sequencing.
Previous hands-on experience with cell automation equipment including automated clone picking, clone consolidation, kinetic imaging systems (Essen Incucyte), bioreactors, wavebags and large flask handling systems such as the SelecT.
Previous experience with human primary cell models and/or iPSCs and iPSC-derived models.
Ability to effectively participate in cross functional collaboration and demonstrate excellent verbal and written communication skills.
Excellent work planning, organization and record keeping is essential.
Ability to multi-task and to balance bench and desk activities.
Ability to perform practical laboratory bench work and work on a computer (while standing or sitting). Ability to perform mathematical calculations and interpret data.
NON-STANDARD WORK SCHEDULE, TRAVEL OR ENVIRONMENT REQUIREMENTS
Minimal, if any travel related to this position. Candidate may periodically be asked to travel to the Pfizer Cambridge, MA site to meet project team members.
Candidate must comply with laboratory safety practices.
EEO & Employment Eligibility
Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.
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Other Job Details:
Last Date to Apply for Job: October 5, 2018
Eligible for Relocation Package: no
Pfizer is an equal opportunity employer and complies with all applicable equal employment opportunity legislation in each jurisdiction in which it operates.