Scientist/Sr. Scientist, In Vivo/Phage Antibody Discovery - Protein Sciences

Surrozen South San Francisco , CA 94080

Posted 2 months ago

Surrozen is a biotechnology company focused on discovering and developing novel regenerative medicines with a focus on unlocking the powerful self-renewal properties of the body through specific control of the Wnt signaling pathway.

Surrozen was founded by five leading-edge scientists: K. Christopher Garcia, Ph.D., Roel Nusse, Ph.D. and Calvin Kuo, M.D., Ph.D. from Stanford University; Claudia Janda, Ph.D. from the Princess Maxima Center for Pediatric Oncology; and Hans Clevers, M.D, Ph.D. from the Hubrecht Institute and Princess Maxima Center. Each has conducted extensive research on Wnt signaling, with findings that together shape Surrozen's technology and approach to therapeutic development.

The Scientist/Sr. Scientist, In Vivo/Phage Antibody Discovery within the Protein Sciences Group is a great opportunity for experienced scientists to lead antibody discovery efforts using technologies including phage display and in vivo antibody discovery with single B cell cloning or immune display techniques to contribute to Surrozen's therapeutic pipeline.

  • Ph.D. in Molecular Biology, Immunology, or related life science field (or equivalent) with 3+ years experience with antibody discovery in biotechnology and/or pharma

  • Experience with molecular biology techniques such as E. coli transformation and cultivation, DNA extraction, enzyme digestion and gel electrophoresis experience

  • Ability to work independently while also contributing to project teams to discover potential therapeutic candidate antibodies as well as discovery antibodies for research related efforts

  • Candidates with hands-on experience with phage display, single B cell cloning, or immune display technologies highly desirable

  • Proficiency working with DNA analysis software programs such as Snapgene or their equivalent and with Microsoft Office (Word, Excel, PowerPoint)

  • Maintain a detailed and well-organized record of experiments in an electronic notebook

  • Preparation of technical reports, summaries and detailed protocols, and contribute to company reports as requested

  • Team player, committed to success and getting the job done well in a highly dynamic environment

  • Troubleshoot instrumentation or software issues and recommend solutions to meet objectives.

  • Strong interpersonal skills

  • Clear and effective written and verbal communication skills

  • Reliable availability for limited weekend work

RESPONSIBILITIES

  • Discover antibodies against challenging targets using phage display technologies, single B cell cloning, and high-throughput screening methods (ELISA/FACS)

  • Work closely with the Antigen Generation Group to design complex immune tolerance breaking strategies and other next generation technologies for in vivo antibody discovery campaigns

  • Work closely with the Protein:Protein Interactions Group to use HTP screening technologies (i.e., Surface Plasmon Resonance and Bio-Layer Interferometry) to identify lead antibody candidates

  • Utilize cloning and PCR methodologies including restriction digests, Gibson assembly, vector and primer design in antibody discovery campaigns

  • Work with other members of Protein Sciences to incorporate automation and liquid handling technologies in antibody discovery campaigns

  • Perform PCR, DNA mini-, midi-, maxi-prepping procedures

  • Maintain and archive DNA clones and bacterial glycerol stocks

  • We offer a dynamic start-up environment and a collaborative, passionate team

  • Excellent benefits, including competitive employer contributions

  • Sock options

  • Paid vacation, sick time and holidays

  • Shuttle service to/from Caltrain, BART and the South San Francisco Ferry

  • State-of-the-art research facility and onsite gym (see the amenities available at The Cove: http://leasing.covessf.com/toc.cfm

icon no score

See how you match
to the job

Find your dream job anywhere
with the LiveCareer app.
Mobile App Icon
Download the
LiveCareer app and find
your dream job anywhere
App Store Icon Google Play Icon
lc_ad

Boost your job search productivity with our
free Chrome Extension!

lc_apply_tool GET EXTENSION

Similar Jobs

Want to see jobs matched to your resume? Upload One Now! Remove
Scientist I Drug Discovery

Tenaya Therapeutics, Inc.

Posted 7 days ago

VIEW JOBS 11/11/2019 12:00:00 AM 2020-02-09T00:00 <p>Tenaya is a growing biopharmaceutical company with a goal to create a new generation of therapies for the treatment of heart disease. We are progressing our reprogramming gene therapy project towards a development candidate and IND filing. We are establishing target and drug discovery technologies including the development of a panel of iPSC-CMs representing human disease-causing mutations. Our mission is to become a preeminent biopharmaceutical company discovering and developing medicines to cure heart failure.</p> <p>We are seeking a creative and detail-oriented scientist with an interest in discovery and development of novel therapies to join our growing team. The successful candidate will work both independently and collaboratively as part of the Drug Discovery team. Hands-on <i>in vitro</i> experience in iPSC culture and differentiation to cardiovascular lineages, and assessment of cardiac pathophysiology is preferred. The scientist will be involved in assay development for drug discovery and mechanistic studies that will contribute to the development of novel therapeutic strategies for the treatment of heart disease. This is a unique opportunity to join an energetic, early-stage company at the leading edge of basic and clinical science.</p><p><br></p> <p><strong>Key Responsibilities:</strong></p> <ul> <li>Lead and work independently to plan, design and execute <i>in vitro</i> experiments to identify and characterize innovative drug therapies for the treatment of heart failure</li> <li>Develop and validate pathophysiological heart disease models including drug-induced, tissue-engineered, and genetically modified cardiac dysfunction models by utilizing the platform of human iPSC-derived cardiomyocytes</li> <li>Drive mechanistic assay development involving molecular biology techniques including plasmid design, nucleic acid and protein isolation, PCR, cloning, virus preparation, and immune assays</li> <li>Generate SOP protocols when required for new procedures</li> <li>Collaborate with biochemists, chemists, and <i>in vivo</i> scientists to advance projects</li> <li>Regularly present scientific findings in internal and external meetings </li> <li>Mentoring and supervision of junior members may be required</li> </ul><p><strong>Requirements</strong></p><p><strong>Skills &amp; Competencies:</strong></p> <ul> <li>Self-motivated and passionate for cutting edge science and technologies</li> <li>Knowledge and expertise in the fields of stem cell and cardiac biology</li> <li>Previous documented and extensive practical experience of cell culture and analyses of cells or tissues derived from the cardiac system</li> <li>Experience of molecular biology and automated cell analyses</li> <li>Ability to collect, analyze, and communicate complex scientific data both verbally and in writing</li> <li>Ability to work productively in a collaborative and fast-paced environment</li> </ul> <p><strong>Education &amp; Experience:</strong></p> <ul> <li>Ph.D. in cardiovascular physiology, cell biology, tissue engineering, or related discipline</li> <li>0 to 2 years work experience in academic or industrial research</li> <li>Strong record in utilizing in-vitro cardiovascular models for study of cardiovascular diseases</li> <li>Proficiency in Graph Pad, Excel, PowerPoint, statistical data analysis</li> </ul><p><br></p><p>Please provide your CV, including list of publications and names and contact info for 3 references, together with a letter of motivation.</p> Tenaya Therapeutics, Inc. South San Francisco CA

Scientist/Sr. Scientist, In Vivo/Phage Antibody Discovery - Protein Sciences

Surrozen