Bighat Biosciences San Mateo , CA 94401
Posted 3 weeks ago
The Role: BigHat is looking for an energetic and motivated individual to onboard and lead cell line engineering as a core Platform capability in support of our pipeline Discovery and early R&D efforts. This role will report directly to the Associate Director, Platform Technology, work cross functionally with Programs and Discovery, and directly impact the identification and validation of next generation biotherapeutics engineered on the Milliner Platform. The Role: BigHat is looking for an energetic and motivated individual to onboard and lead cell line engineering as a core Platform capability in support of our pipeline Discovery and early R&D efforts. This role will report directly to the Associate Director, Platform Technology, work cross functionally with Programs and Discovery, and directly impact the identification and validation of next generation biotherapeutics engineered on the Milliner Platform.
Key Responsibilities
Onboard all the core components necessary for engineering cell lines expressing pipeline targets including expression vectors, gene delivery methods, selective antibiotic concentration determination protocols, expression validation means, and cell sorting capabilities
Culture and maintain a variety of cell types; generate diverse target cell lines including knock-outs, cells expressing multiple targets, and reporter cell lines
Perform the validation of cell line expression using FACS and take on bulk pool and single cell sorting to select for cell lines of various expression levels
Maintain an organized cell line inventory with all relevant documentation
Utilize literature and external resources as necessary to investigate target expression biology to recapitulate the most native conditions for expression.
Work with end user stakeholders to confirm correct domain expression and associated sequences
In collaboration with end user stakeholders, establish the workflow,timelines and deliverables for cell line engineering from request to hand-off
Skills Knowledge and Expertise
PhD in biology-related discipline with 2+ years industry experience, preferably in an environment utilizing cell lines for antibody discovery and engineering
Molecular biology knowledge for plasmid construction and experience with various expression vector and promoter types
Experience culturing various cell types, including adherent, suspension, shaker flask etc. High level of organization in handling multiple cell lines simultaneously for manipulation and maintenance
Demonstrated hands-on success in using CRISPR, electroporation, and lentivirus methods for enabling knock-out and expression
Adept with FACS, cell sorting methods, and clonal cell selection and maintenance
Prior use of automation for cell culture a plus
Bighat Biosciences