Scientist / Senior Scientist, Cell Biology

Generation Bio Cambridge , MA 02142

Posted 3 weeks ago

Generation Bio is innovating genetic medicines to provide durable, redosable treatments for people living with rare and prevalent diseases. The companys non-viral platform incorporates a novel DNA construct called closed-ended DNA, or ceDNA; a unique cell-targeted lipid nanoparticle delivery system, or ctLNP; and a highly scalable capsid-free manufacturing process that uses its proprietary cell-free rapid enzymatic synthesis, or RES, to produce ceDNA. The platform is designed to enable multi-year durability from a single dose, to deliver large genetic payloads, including multiple genes, to specific tissues, and to allow titration and redosing to adjust or extend expression levels in each patient. RES has the potential to expand Generation Bios manufacturing scale to hundreds of millions of doses to support its mission to extend the reach of genetic medicine to more people, living with more diseases, around the world.

We are a thriving, collaborative, creative community of 140 people with diverse talents, beliefs, and experiences headquartered in Cambridge, Massachusetts.

Delve further. Please visit www.generationbio.com or follow @generationbio.

SUMMARY:

Generation Bio is seeking an energetic and highly motivated Scientist/Senior Scientist to join the Cell Biology team within the Platform Research group. The position will play a key role in development and performance optimization of our non-viral gene therapy platform by developing and executing experiments aimed at deciphering the cell biological process involved in ctLNP uptake, endosomal escape and intra-cellular ceDNA transport. The ideal candidate will have a strong background in development and implementation of assays deciphering and manipulating the cellular immunological responses to ceDNA vector delivery, epigenetic modulation of ceDNA and assessment of its long-term expression in vitro and in vivo. The position will move seamlessly between leading lab-based work to design/implement assays in our state-of-the-art cell and molecular biology laboratories, providing scientific leadership roles in cross functional working groups and managing external studies with CROs and collaborators.

RESPONSIBILITIES:

  • Work in cross functional research teams to optimize ctLNP/ceDNA platform elements for desired long-term expression and immunologic profile of ceDNA
  • Design and implement molecular biological tools like genome editing in vitro and in vivo
  • Design, optimize and execute in vitro/in vivo assays to investigate potential immunogenic cell-autonomous and cell non-autonomous responses to cytoplasmic and nuclear ceDNA
  • Develop and implement in vitro/in vivo assays to assess epigenetic modulations of ceDNA and their effects on long-term stability and expression
  • Support development and evaluation of in vivo tools in appropriate animal disease models
  • Summarize studies in written reports and oral presentations
  • Interface externally with collaborators, and in management of research performed by CRO(s)

QUALIFICATIONS:

  • Ph.D. in Virology, Molecular/Cell Biology, Immunology, Pharmacology or related field; minimum of 2 years of post-PhD experience preferred
  • Experience in viral/non-viral gene therapy or a background in virology/ antiviral immunology highly desired
  • Excellent lab technique and the ability to work independently to design experiments and analyze, interpret, and present data required
  • Highly proficient in
    • Cell culture (cell lines, primary cells) and cellular phenotypic and activity assays
    • Genome editing assays
    • Protein and DNA/RNA detection and quantification (RT-qPCR, (sc)RNAseq, ELISA, Western Blot, MSD, Octet)
    • Epigenetic analysis assays (ChIP-seq, ATAC-Seq, Bisulfite sequencing)
    • Cell-based high-content imaging (confocal imaging, IHC, flow cytometry, RNA/DNA (F)ISH)
  • Demonstrated leadership capabilities in cross-functional integration and management
  • Proactive thinking and ability to multi-task to support multiple projects across platform and disease biology pharmacology
  • Ability to work in a highly collaborative, team-based environment a must
  • Excellent written and verbal communication skills

POSITION: Full-Time, Exempt

EEOC Statement: Generation Bio believes in a diverse environment and is committed to equal employment opportunity for all its employees and qualified applicants. We do not discriminate in recruitment, hiring, training, promotion or any other employment practices for reasons of race, color, religion, gender, national origin, age, sexual orientation, marital or veteran status, disability, or any other legally protected status. Generation Bio will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.

icon no score

See how you match
to the job

Find your dream job anywhere
with the LiveCareer app.
Mobile App Icon
Download the
LiveCareer app and find
your dream job anywhere
App Store Icon Google Play Icon
lc_ad

Boost your job search productivity with our
free Chrome Extension!

lc_apply_tool GET EXTENSION

Similar Jobs

Want to see jobs matched to your resume? Upload One Now! Remove
Molecular And Cell Biology Scientist

Eurofins Scient.

Posted 3 weeks ago

VIEW JOBS 9/23/2021 12:00:00 AM 2021-12-22T00:00 Eurofins Scientific is an international life sciences company, providing a unique range of analytical testing services to clients across multiple industries, to make life and our environment safer, healthier and more sustainable. From the food you eat, to the water you drink, to the medicines you rely on, Eurofins works with the biggest companies in the world to ensure the products they supply are safe, their ingredients are authentic and labelling is accurate. Eurofins is the global leader in food, environment, pharmaceutical and cosmetic product testing and in agroscience Contract Research Organisation services. Eurofins is one of the market leaders in certain testing and laboratory services for genomics, discovery pharmacology, forensics, advanced material sciences and in the support of clinical studies, as well as having an emerging global presence in Contract Development and Manufacturing Organisations. The Group also has a rapidly developing presence in highly specialised and molecular clinical diagnostic testing and in-vitro diagnostic products. In over just 30 years, Eurofins has grown from one laboratory in Nantes, France to 55,000 staff across a decentralised and entrepreneurial network of 900 laboratories in over 50 countries. Eurofins offers a portfolio of over 200,000 analytical methods to evaluate the safety, identity, composition, authenticity, origin, traceability and purity of biological substances and products. In 2020, Eurofins generated total revenues of EUR € 5.4 billion, and has been among the best performing stocks in Europe over the past 20 years. * Execution of a limited number of assays for the analysis of routine samples using dissolution methods, HPLC/UPLC, spectroscopic and compendial methods. * Basic lab organization and maintenance * Identify and help to resolve equipment and basic test method issues * Develop expertise to serve as a backup executor of a limited number of other analysis types (other test methods) * Provide feedback to client on the performance of existing assays / test methods * Excellent documentation skills * Master's degree in science (chemistry, pharmacy, or equivalent life sciences degree) or * Prior knowledge and experience with the dissolution methods, HPLC/UPLC, UV/vis spectroscopy, SOTAX automation platforms and/or compendial test methods (pH, osmolality, HIAC) * Basic lab organization and maintenance skills * Excellent documentation skills * Strong attention to detail * Ability to work independently and as part of a team, self-motivation, adaptability, and a positive attitude Position is full-time, Monday - Friday 8:00am - 5:00pm. Candidates currently living within a commutable distance of Cambridge, MA are encouraged to apply. Excellent full time benefits including comprehensive medical coverage, dental, and vision options * Life and disability insurance * 401(k) with company match * Paid vacation and holidays Eurofins is a M/F, Disabled, and Veteran Equal Employment Opportunity and Affirmative Action employer. Eurofins is a M/F, Disabled, and Veteran Equal Employment Opportunity and Affirmative Action employer. Eurofins Scient. Cambridge MA

Scientist / Senior Scientist, Cell Biology

Generation Bio