Support neuroscience research focused on ion channel function and psychiatric disease using a variety of laboratory techniques, including molecular and cellular neurobiology, electrophysiological recordings, and imaging. Work under minimal supervision to design and execute experiments.
Generate mutations found in patients by utilizing cloning, transfection, electroporation, and viral transduction, and examine its impact on ion-channel function by automated patch-clamp recording and high-throughput calcium influx assay. Work with disease models acquired from human embryonic stem-cell driven neurons, primary cultured neurons of transgenic mice and genetically knockdown primary cultured neurons using RNA interference. Assess candidate compound molecules for potential therapeutic effects on multiple functional endpoints that will be discovered by successful candidate.
Examine neuronal firing by optical imaging of neuronal activity and by manual patch-clamp recording. Examine neuronal network activity by microelectrode-array and brain-slice electrophysiology. Examine morphological feature of neuronal network by quantifying synapse number and dendrite outgrowth.
Utilize immunofluorescence-imaging techniques such as immunocytochemistry, immunohistochemistry, and confocal microscopy imaging as required. Perform high-content imaging. Quantify protein expression of target gene by western blotting and by immunofluorescence imaging techniques.
Support gene expression study such as qRT-PCR, qRT-PCR array, and RNA sequencing. Conduct luciferase reporter assay to analyze transcription activity. Responsible for primary neuronal cell culture and conventional cell culture.
Learn new techniques and approaches as drug discovery and development program progresses. Create and maintain careful records of experiments and findings. Communicate findings in written and verbal reports as necessary.