Scientist I, Flow Cytometry

TITLE : Scientist I, Flow Cytometry

REPORTING LOCATION: Rockville, MD

PRIMARY OBJECTIVES: Support the pre-clinical and clinical teams with exceptional immunology research skills

DUTIES AND RESPONSIBILITIES

• Perform multi-parameter flow cytometry including panel design (>12 colors), instrument set-up, sample acquisition and data analysis

• Generate novel methods and/or work with method developers to develop, transfer, and validate appropriate flow cytometry assays

• Perform ex vivo and in vitro assays for immuno-oncology relevant endpoints such as ELISA and multiplex cytokine assays (e.g. Luminex and MSD)

• Responsible for the quality and efficiency of studies performed within the pre-clinical and clinical laboratory.

• Project management: lead analysis of pre-clinical and clinical flow cytometry data (using Flowjo, FACS Suite and Diva software) and reporting of results.

• Responsible for training internal staff or external collaborators on scientific processes

• Responsible for scheduling and planning to effectively manage workload

• Ensuring compliance with established procedures and environment in a regulated laboratory.

• Ability to interact directly with customers

Qualifications

KNOWLEDGE, SKILLS, AND ABILITIES

• Proficient in multi-parameter flow cytometry including panel design (>12 colors, instrument set-up, sample acquisition, data analysis)

• Strong data analysis experience using Flowjo, Diva software and other FACS related analysis tools

• Experience with PK/PD, in vitro T-cell functional characterization assays including in vitro cytotoxicity, proliferation, and cytokine/chemokine measurements.

• Proficiency in the analysis and understanding of immune cells and molecules.

• Perform ex vivo and in vitro assays for immuno-oncology relevant endpoints (e.g. ELISA and multiplex cytokine assays)

• Preparation and handling of primary specimens, including peripheral blood, ascites, other liquid biospecimens.

• Primary immune cell culture (experience with T cells, NK cells, Dendritic cells, Macrophages)

• Sample preparation (murine or human tissues/cells) for immunostaining and acquisition by flow cytometry

• Troubleshoot, optimize, and develop methods for unmet experimental needs

• Maintain laboratory records and notebook, organize and compile data, and write weekly reports on results

• Present data, compose manuscripts & prepare posters/presentations at national/international meetings or symposia in support of business objectives

• Experience in data interpretation and statistical analysis.

• Train internal staff or external collaborators on scientific processes.

• Manage timelines and deliverables and develop reports for various projects.

• Coordinate parallel tasks across multiple projects, demonstrating prioritization.

• Strong planning, organizational, computer, written and verbal communication skills.

• Demonstrated accountability and responsibility for own work.

• Capable of high performance in independent work as well as in team setting.

EDUCATION AND EXPERIENCE

• Ph.D. in immunology, cellular and/or tumor biology or a closely related field (0-3 year of experience)

• Must have experience in flow cytometry, immune cell phenotyping of immune cell subsets

• Experience designing and developing flow cytometry panels for pre-clinical and clinical samples

• Demonstrated experience with in vitro assay development preferred

• Demonstrated experience working with pre-clinical models preferred

• Demonstrated experience in in vivo immunology and oncology tumor model prefer

PHYSICAL DEMANDS AND WORK ENVIRONMENT

• Must be able to sit and use hands to finger, handle and feel.

• Must be able to lift and move packages weighing up to 50 pounds.

• Must be able to sit for long periods of time using a computer in a typical office environment in a multi-level facility

• Some activities require repetitive motion and reaching, pulling or pushing.

• In carrying out the duties of the job there will be frequent exposure to fumes and chemical hazards.

• Duties performed routinely require exposure to blood, body fluid and tissue.

This description reflects management's assignment of essential functions, it does not proscribe or restrict the tasks that may be assigned. We celebrate diversity and are committed to creating an inclusive environment for all employees.