GENERAL SUMMARY/ OVERVIEW STATEMENT:
The goal of Dr.
Phillippe's many years of funded research has been to elucidate the cellular and cell-signaling mechanisms within the pregnant uterus that underly the onset of labor (parturition). Currently, research in Dr.
Phillippe's laboratory is addressing various aspects of a possible telomere-based biologic clock mechanism that could determine the length of pregnancy and the timing for the onset of parturition. These studies are assessing the shortening of telomeres in gestational tissues (i.e. the placenta and fetal membranes) of pregnant mice and women, the relationship between apoptosis of gestational tissues and the release of cell-free DNA, and the ability of cell-free DNA released by these tissues to stimulate an innate immune response through activation of Toll-like receptor-9. Dr.
Phillippe's current research utilizes in-vitro cell cultures, placental and fetal membrane explant cultures, in-vivo timed-pregnant mouse models and archived human gestational tissues along with various molecular techniques to assess the role of telomere shortening, oxidative stress, apoptosis, and innate immunity occurring during onset of parturition both at term and preterm.
Because of the tremendous clinical and biologic importance of clarifying the mechanism(s) that determine the length of pregnancy and the timing for the onset of labor, this area of research will remain the focus for Dr.
Phillippe's laboratory for the foreseeable future.
The Senior level Research Technologist will report directly to the Principal Investigator, Lab Director, and/or managers of the laboratory and is expected to work independently on assigned research with minimal guidance from a higher level staff member or Principal Investigator. The Senior Technologist may also serve as team leader to other lab assistants and Research Technicians I and II, if applicable.
The ideal Senior Technologist is expected to have extensive experience in mouse models and handling, in particular retrieval/dissection of gestational and other tissues, preservation for RNA, DNA and immunohistochemical analyses, and in molecular techniques including qPCR, FISH, ELISAs, and more. The Senior Technologist is expected to lead a research project to assess various aspects and effects of telomere shortening during aging of the gestational tissues primarily using pregnant mouse and cellular models in a biomedical research laboratory. With broad guidance from the Principal Investigator, the Senior Technologist is expected to independently design and perform experiments to accomplish laboratory research goals.
In addition, she/he will be responsible for documenting, analyzing, and reporting the results derived from mouse models and/or patient samples. The Senior Technologist will assist in the preparation of manuscripts for publication; and she/he will also assist with gathering information needed for grant preparation and reporting. She/he must be proficient in basic computer skills including word-processing, database and spreadsheet management, email and internet.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Primary responsibility of this role will be performing experiments in a biomedical research laboratory for NIH- and foundation-funded research, studying the effects of aging of gestational tissues as a biologic clock mechanism that determines the length of pregnancy and the timing for the onset of parturition. The position requires a team-based approach between the PI and Senior Technologist where the Technologist's input into experimental designs and protocols, and recommendations for innovation, will be highly valued.
Carries out a wide range of moderate to complex lab techniques such as, but not limited to, ELISAs, DNA extraction and quantitative assays, DNA and protein electrophoreses, qPCR and RT-PCR studies, small animal surgery, mouse colony maintenance, histology, histochemistry, and tissue/cell culture work. Independently modifies techniques as necessary to meet research and/or experimental needs.
Collects and records study data. Synthesizes and interprets information obtained from graphs, charts, laboratory experiments, statistical summaries and other data collection methods.
Interprets results to develop new investigative techniques and/or to determine the most appropriate direction of research. Synthesis may involve use of computer programs as well as data entry into databases.
Participates in the design of experiments and performs procedures (i.e. cell and tissue culture). Develop new experimental protocols, execute protocols of non-routine experiments, troubleshoot experimental problems and provide alternative approaches.
She/he is expected to design and conduct experiments independently in the laboratory with general guidance from the Principal Investigator, and participate in the design of complex experiments, including the design and modification of current protocols, the execution protocols of non-routine experiments, and to assists the Principal Investigator with the determination of the most optimal methodologies.
Maintains an electronic laboratory notebook (LabArchives). Prepares written reports on status of research, determination of technical procedures, sequence of operations, including statistical and graphic summaries of findings and possible conclusions. Collaborates with supervisor in writing and editing material for publication and may appear as principal or secondary author on publications
Organizes and drafts materials for publication. Independently collect and frequently analyze experimental results using appropriate statistical analysis, including judging the validity and reliability of experimental data, summarizing research results, preparing written progress reports and performing independent literature searches.
May operate and maintain moderate to complex lab equipment such as PCR and real-time qPCR machines, 96-well plate washers and readers, fluorescence and light microscopes, chemiluminescence and UV digital image analyzer and other scientific equipment related to research activity.
Participates in the upkeep of the lab; this includes keeping both direct and general work spaces clean and tidy and helping maintain an overall safe and clean environment. May perform, or refer as needed, routine maintenance of lab equipment to include cleaning and replacing of parts. Report equipment malfunctions and needs to the laboratory supervisor.
Maintains and orders supplies; and keep an accurate inventory of laboratory supplies and reagents.
Work safely within the lab environment and in performing work duties. Wear personal protective equipment as required.
Attend and actively participate in laboratory meetings, seminars and journal clubs. Prepares and presents reports for lab meetings.
SKILLS/ ABILITIES/ COMPETENCIES REQUIRED:
The Senior Technologist should have a strong background in molecular biology, biochemistry, genetics and reproductive biology; and should understand theories behind assays and procedures and have the ability to identify problems and develop solutions.
The Technologist should be able to work independently and display initiative to introduce new methodologies to research study.
The Technologist should have comprehensive knowledge of experimental animal care and handling, especially handling and breeding of mice, and surgery/tissue retrieval from embryos, juvenile and adult offspring generated in the colony.
Ability to prepare IACUC and IRB protocols and maintain compliance with required periodic documentation.
Ability to prioritize tasks and meet deadlines.
Sound interpersonal skills and the ability to train and supervise junior laboratory members.
Excellent oral and written communication skills.
Computer competency is required, along with knowledge of Microsoft Office and statistical analytical software (e.g. SigmaStat, Graphpad Prism, etc.).
Is knowledgeable and compliant in all hospital, State and Federal regulatory requirements, including hospital policy and procedures (where applicable to the performance of the job), Joint Commission on Accreditation of Healthcare Organizations (JCAHO), and Health Insurance Portability and Accountability Act of 1996 (HIPAA).
LICENSES, CERTIFICATIONS and/or REGRISTRATIONS (if applicable):
SUPERVISORY RESPONSIBILITY (if applicable):
The Senior Technologist will be expected to provide necessary research support for other researchers and assist the training of students, postdocs and clinical fellows working in Dr.
FISCAL RESPONSIBILITY (if applicable):
Duties will be performed in a research laboratory. This setting may create contact or exposure to one or more of the following: laboratory animals (including mice), human tissues, chemicals, fluids, sharp instruments and other supplies and equipment consistent with a research lab. Contact or exposure may be airborne or physical.
Work will be conducted over a forty-hour workweek, but actual schedule can be flexible
BS/BA degree in biomedical science or a BS/BA in any discipline with significant formal biomedical research training is required. Higher degree including M.S. or PhD degree is preferred but not required, depending on experience.
Three (3) or more years of experience and/or training related to biomedical research is required.
Two (2) or more years of experience working with rodent models is required, including experience with small animal breeding and non-survival surgery for tissue harvest.
Previous laboratory experience in molecular biology techniques and tissue/cell culture experience is required
Partners Healthcare System