Research Technician, Radiation Oncology

GENERAL SUMMARY/ OVERVIEW STATEMENT: Summarize the nature and level of work performed.

Oncology Lab Research Technician

A research technician position is available in the laboratory of oncologist Dr. Andrew Elia at Harvard Medical School and Massachusetts General Hospital Cancer Center.

The candidate will assist in characterizing DNA damage response pathways necessary for maintaining genomic stability and thereby preventing cancer. This work will also focus on elucidating pathways involved in DNA damage activation of immune signaling. Overall, these studies will help to find new targets for the treatment of tumors with specific DNA repair defects and will help to improve the efficacy of combining genotoxic cancer treatments with immunotherapy.

Principal investigator has strong track record of technicians placing into top medical and graduate school programs. Graduating seniors are encouraged to apply. Position will commence between May - August 2024.

PRINCIPAL DUTIES AND RESPONSIBILITIES: Indicate key areas of responsibility, major job duties, special projects and key objectives for this position. These items should be evaluated throughout the year and included in the written annual evaluation.

Duties and Responsibilities:

1.Characterize novel proteins involved in DNA damage and immune signaling pathways using cell biology and biochemistry techniques (CRISPR, RNAi, western blotting, immunofluorescence, immunoprecipitation, chromatography, flow cytometry, and reporter gene assays).

2.Perform standard molecular biology techniques (cloning, site-directed mutagenesis, production and purification of recombinant proteins).

3.Perform tissue culture work doing plasmid transfection, retroviral transduction, and lentiviral transduction.

4.Assist in CRISPR functional genomic screen to identify genes required for the selective survival of tumor cells with specific DNA repair or immune signaling defects.

5.Assist with quantitative mass spectrometry proteomics to identify novel post-translational modifications involved in the DNA damage response.

6.Assist with cancer gene mouse model.

7.Establish new and modify existing research techniques.

SKILLS/ABILITIES/COMPETENCIES REQUIRED: Must be realistic, objective, measurable and related to essential functions of this job.

Self-motivated, excited by science and medicine

Ability to perform as a team member

Independent thinker who takes ownership of work

Meticulous attention to detail

Excellent organizational and analytical skills

Committed to producing accurate and high-quality work

LICENSES, CERTIFICATIONS, and/or REGISTRATIONS (if applicable): Specify minimum credentials and clearly indicate if preferred or required

EDUCATION: Specify minimum education and clearly indicate if preferred or required

Bachelor degree in biology, biochemistry, chemistry, biotechnology or related science field

EXPERIENCE: Specify minimum creditable years of experience and clearly indicate if preferred or required

1-3 years experience in a molecular laboratory preferred.