Research Lab Tech I, Immunology Research, Division Of Trauma, Burn, & Surgical Critical Care

This position is for a technical research assistant with dual roles. The first role is to conduct basic cellular immunology research using mass cytometry (CyTOF) to characterize and phenotype human and mouse immune cell subsets.

The second role is to assist running the CyTOF Antibody Resource and Core that serves the Harvard Medical Area community. These two roles will be accomplished simultaneous with the technician splitting their time equally between both roles.

The technician will work on studies to profile human blood immune cells from patients with a variety of pathologies, including but not limited to autoimmunity, cancer, and traumatic injury. Mouse disease model studies will also be used to study immune cell responses to injury, infections, and radiation. The technician will work in tandem with another technician on day-to-day operations of the CyTOF antibody resource.

The technician's daily activities will involve validating and testing CyTOF antibody panels, cell preparation, cell staining with CyTOF antibodies, and data analysis. The technician will also help order supplies, prepare specialized reagents, and assist others in the lab or collaborators with CyTOF technology, animal research work, and other associated experimental techniques.

PRINCIPAL DUTIES AND RESPONSIBILITIES:

1.Help run the CyTOF Antibody Resource and Core - conjugating antibodies, validating conjugations, fulfilling orders, troubleshooting user requests, and participate in scientific collaborations with Core users (leading to publications)

2.Process human and mouse tissue into cell suspensions for experiments and to validate CyTOF-ready antibodies

3.Consult and interact with CyTOF users on the technical features of the technology

4.Analyze CyTOF staining data using software for multidimensional cell staining data

5.Train others in the laboratory on using CyTOF technology and other techniques

6.Work as a team on specific studies to profile human and mouse blood cells or tissue cells to identify disease-specific phenotypes.

QUALIFICATIONS:

1.BS or BA required, concentration in biology, microbiology, chemistry, or related biological sciences.

2.0-2 years of practical experience in the field

SKILLS/ ABILITIES/ COMPETENCIES REQUIRED:

1.Basic laboratory skills

2.Computational skills and knowledge of how to use R programming or similar platform

3.Organizational skills and ability to focus on details

4.Good interpersonal and communication skills

5.Ability to demonstrate professionalism

HOSPITAL WIDE RESPONSIBILITIES:

Works within legal, regulatory, accreditation and ethical practice standards relevant to the position and as established by BWH/MGB; follows safe practices required for the position; complies with appropriate BWH and Partners policies and procedures; fulfills any training required by BWH and/or MGB, as appropriate; brings potential matters of non-compliance to the attention of the supervisor or other appropriate hospital staff.

The above is intended to describe the general contents and requirements of work being performed by people assigned to this classification. It is not intended to be construed as an exhaustive statement of all duties, responsibilities or skills of personnel so classified.