Research Associate II, In Vivo Pharmacology (Contract)

The Organization

What's happening at Entrada Therapeutics? Entrada is a clinical-stage biopharmaceutical company aiming to transform the lives of patients by establishing a new class of medicines that engage intracellular targets that have long been considered inaccessible.

Our Endosomal Escape Vehicle (EEV)-therapeutics are designed to enable the efficient intracellular delivery of a wide range of therapeutics into a variety of organs and tissues, resulting in an improved therapeutic index. Through this proprietary, versatile and modular approach, Entrada is advancing a robust development portfolio of RNA-, antibody- and enzyme-based programs for the potential treatment of neuromuscular, ocular, metabolic and immunological diseases, among others.

Our lead oligonucleotide programs are in development for the potential treatment of people living with Duchenne who are exon 44, 45 and 50 skipping amenable. We have also partnered to develop a clinical-stage program, VX-670, for myotonic dystrophy type 1.

We are a tight-knit team of experts and leaders in both therapeutic development and rare diseases and are excited to grow and attract colleagues who are ready to join a high-energy, dedicated team that likes to get things done and dramatically improve the lives of patients and their families.

The Perfect Addition to Our Team

The In Vivo Pharmacology contractor will primarily be focused on contributing to the execution of in vivo experiments, directly fulfilling company goals, and validating our modular intracellular delivery platform. This position will create exposure to in vivo techniques and animal models, ex vivo assays, as well as the opportunity to participate in cutting edge science.

This position offers a long-term contract opportunity with Entrada. The individual will be based on-site 5 days per week in our Seaport office.

The Opportunity

Entrada is hiring a highly motivated in Vivo Pharmacology contractor to support the Company's intracellular oligonucleotide targeted delivery and biologics discovery and development efforts. The successful candidate will work closely with a highly engaged, talented In vivo scientist including In vitro Pharmacology team members. The successful candidate is expected to be involved in all aspects of early concept/Discovery pre-clinical in vivo studies including dosing, perfusion, tissue collection and cryo-pulverization of tissue samples. Furthermore, the successful applicant will be working under the supervision of In vivo team members for execution and completion of pre-clinical PoC studies upon completion of training period. As part of the Pharmacology functional team, this role could contribute broadly to the mission of advancing validated targets into the drug discovery portfolio pipeline.

Responsibilities

• Perform various in vivo routes of administration in rodents specifically proficient in tail vein intravenous dosing, including but not limited to, subcutaneous, intraperitoneal, intramuscular, and intranasal.

• With training and supervision, carry out all workflow process of in vivo pharmacology to support studies, including animal handling, dosing, bleeding, necropsy, tissue collection and wet lab sample processing.

• Perform in vivo tumor efficacy studies including subcutaneous and disseminated tumor cell implantation and longitudinal tumor volume measurement.

• Perform rodent dissection and tissue microdissection.

• Perform basic animal handling and restraint following animal welfare rules and regulations.

• Assess and monitor rodent health.

• Collect and document important in-life data points from rodents.

• Participate in the processing and analysis of various tissue types across multiple rodent species.

• Support and participate in basic ex vivo assays.

• Perform aseptic cell culture and primary cell isolation and maintenance.

• Demonstrate a high level of initiative to troubleshoot and accurately perform data analysis and present experimental data to internal groups.

The Necessities

At Entrada, our passion for science, our devotion to patients and our values drives our behavior:

• Humanity

• We genuinely care about patients and about one another.

• Tenacity

• We are relentless and persistent in the pursuit of developing therapies for patients.

• Creativity

• We are creative problem solvers.

• Collaboration

• We are more than the sum of our parts.

• Curiosity

• We have a growth mindset and push conventional thought and theory.

To thrive on our team, you will need to come with:

• BS or MS with 2 + years of relevant experience in areas related to Cell Biology, Molecular Biology and/or Pharmacology in an industrial/CRO or academic setting.

• Experience working with rodents, administering doses through various methods including but not limited to PO, IP, IV, SC and IM, and conducting post-study processes including blood draws, necropsies, and ex vivo sample analysis.

• Excellent organization and planning skills, the ability to work in a demanding environment independently or as part of a team, strong motivation with a dedicated work ethic.

• Experience with Contract Research Organizations (CROs) is an asset.

• Experience running flow, ICC, FISH, and/or IHC imaging assays is a plus.

• Prior multicolor flow cytometry, ELISA or other methods of cytokine/analyte analysis preferable but not mandatory.

• Hands-on experience with a range of in vitro/ ex vivo techniques (such as RNA/DNA extraction, RT-qPCR, ELISA, protein analysis via Western blot, WES, and/or MSD) is desirable.

• A passion for contributing to a team-oriented, diverse, and inclusive work environment.

• Excellent multitasking abilities to thrive in a fast-paced, collaborative setting.

• Prior experience or knowledge of cancer biology, immunology and understanding of in vivo models used in immuno-oncology preferable but not mandatory.

• Software knowledge: Windows, MS Office (Outlook, Word, Excel), GraphPad Prism, FlowJo, Benchling.

• Strong attention to detail and ability to follow protocols and lab procedures.

• Strong written and verbal communication skills and ability to work effectively, independently, and collaboratively in a fast-paced multidisciplinary team environment. #LI-JF1

The Perks

By becoming a team member here at Entrada, you'll have access to competitive health, dental, and vision coverage, as well as life insurance, and short term and long-term disability insurance. We value work life balance, you'll benefit from discretionary time off, paternity leave, and an excellent 401(k) package. We also offer a generous transportation stipend to commute as you wish. Our location in the vibrant and growing Seaport District is close to all the best that the city of Boston has to offer.

Entrada Therapeutics is an equal opportunity employer. Qualified candidates will receive consideration for employment without regard to race, color, religion, national origin, gender, sexual orientation, gender identity or expression, age, mental or physical disability, and genetic information, marital status, citizenship status, military status, protected veteran status or any other category protected by law.

Third Party Staffing Agencies

Entrada does not accept unsolicited resumes from any source other than directly from candidates. For the protection of all parties involved in the recruiting process, resumes will only be accepted from recruiters/agencies if a signed agreement is in place at the inception of the recruiting effort and authorized for a specified position. Unsolicited resumes sent to Entrada from recruiters/agencies do not constitute any type of relationship between the recruiter/agency and Entrada and do not obligate Entrada to pay fees if we hire from those resumes.

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