Research Associate II, Flow

REPORTING LOCATION: Rockville, MD

PRIMARY OBJECTIVES: Support the pre-clinical and clinical teams.

DUTIES AND RESPONSIBILITIES

• Preparation and handling of primary specimens, including peripheral blood, ascites, other liquid biospecimens.

• Isolation of white blood cells by density gradient separation

• Primary immune cell culture (experience with T cells, NK cells, Dendritic cells, Macrophages)

• Sample preparation (murine or human tissues/cells) for immunostaining and acquisition by flow cytometry

• Cell culture for primary cells and cell lines

• Perform multicolor flow cytometry including autonomous use of flow cytometer(s) and flow analysis software

• Perform ex vivo and in vitro assays for immuno-oncology relevant endpoints (e.g. ELISA and multiplex cytokine assays)

• Troubleshoot, optimize, and develop methods for unmet experimental needs

• Maintain laboratory records and notebook, organize and compile data, and write weekly reports on results

• Ability to work Tuesday - Saturday schedule.

Qualifications

KNOWLEDGE, SKILLS, AND ABILITIES

• Expertise in aseptic techniques for handling primary human cells and for mammalian cell culture

• Proficiency with processing primary tissues and other samples for downstream assays

• Proficiency in Immunostaining, flow Cytometry acquisition and analysis

• Good documentation practices and able to follow established standard operating procedures (SOPs)

• Experience working in a regulated environment (GLP or GCLP)

• Proficiency in MS office suite, including Excel, Word and PowerPoint

• Ability to coordinate parallel tasks across multiple projects, demonstrating prioritization.

EDUCATION AND EXPERIENCE

• BS with 2-year experience or MS degree. Preferred field of study in immunology or related sciences.

• Industry experience working with pre-clinical and clinical samples is preferred.

• Experience with multicolor flow cytometry is required

PHYSICAL DEMANDS AND WORK ENVIRONMENT

• Must be able to sit and use hands to finger, handle and feel.

• Must be able to lift and move packages weighing up to 50 pounds.

• Must be able to sit for long periods of time using a computer in a typical office environment in a multi-level facility

• Some activities require repetitive motion and reaching, pulling or pushing.

• In carrying out the duties of the job there will be frequent exposure to fumes and chemical hazards.

• Duties performed routinely require exposure to blood, body fluid and tissue.

This description reflects management's assignment of essential functions, it does not proscribe or restrict the tasks that may be assigned. We celebrate diversity and are committed to creating an inclusive environment for all employees.