Research Associate I / II / III - Hoffmann Lab

Category:

Science

Lab/Area:

Hoffmann Lab

Description:

Who we are:

The Gladstone Institutes is an independent, not-for-profit research institution. Its mission is to improve the health and well-being of all people through medical research and education in neuroscience, cardiovascular biology, immunology, and stem cell biology. Home to notable leaders in science and biomedicine, Gladstone comprises five institutes as well as various centers and programs. All of its approximately 600 members are aligned in their mission to carry out or support science that overcomes disease. Located in an award-winning building at the Mission Bay Campus, Gladstone offers a competitive salary and benefits program. It provides an ideal environment for those seeking to maximize and fully leverage their potential. Consider Gladstone and make a difference in human health.

About the role:

The Hoffmann Lab at the Gladstone Institute of Virology is seeking a motivated individual specializing in, in vivo research to join our dynamic team as a Research Associate. The successful candidate will play a crucial role in supporting our multidisciplinary research efforts to develop our mRNA- and nanoparticle-based vaccine technologies against a wide range of infectious diseases. The selected candidate will be responsible for conducting research in mice to evaluate vaccine candidates, including ordering and maintaining mouse cohorts, immunization, bleeding, harvesting tissues, and general care of the animals. They will work together with members of the lab to develop immunization schedules and schemes and will carry out the immunization experiments and analysis. This position also involves biochemical characterization of the immune responses including ELISAs, in vitro neutralization assays, cloning, and protein purification.

What you will do:

• Planning and conducting laboratory animal experiments in mice including ordering, management and coordination of multiple projects, scheduling, communication with researchers regarding experimental plans and progress status, monitoring animal health status, maintaining facility supplies, vaccine formulation, immunization via various routes (IV, IM, SQ, IN, IP), anesthetic solution formation, anesthesia (inhalant, ip), bleeding, measuring body weight, tissue harvest, euthanasia, serum preparation, single cell preparation for splenocytes

• Molecular biology tasks including cloning (PCR, gel electrophoresis, sequencing and validating constructs, etc.) and plasmid preparation

• Transfection and maintenance of mammalian cell lines

• Protein and VLP purification (affinity and size exclusion chromatography)

• Western blotting, SDS-PAGE, ELISAs, and other assays

• General lab duties including buffer and reagent preparation

• Maintaining accurate records and organized records of experimental procedures and results

• Collaborate with team members on various research projects

• Other duties as assigned

What you will need:

• BA/BS or MS/MA in Biology or related field

• At least two years of experience in a biology research laboratory

• At least two years of extensive hands-on experience with handling of and research activities in mice

• Some molecular biology experience

• Outstanding communication skills, attention to detail, organizational skills, and ability to work on several projects in parallel as part of a team

What is preferred:

• A strong background in immunology, virology, and molecular biology

• Extensive experience conducting vaccine, virology, or immunology research in mice

• Extensive experience in mammalian cell culture and molecular biology (cloning, protein expression and purification, Western blotting, SDS-PAGE, ELISAs, etc.)

• Strong interpersonal skills and the ability to work effectively with individuals from diverse backgrounds

If this role is not for you:

Please stay in touch and to see a complete list of our open career opportunities, please visit:

https://gladstone.org/careers

Hiring Range:

RA I $25-$29/hr

RA II $66,560- $78,306K

RA III $72,914 - $85,782

Gladstone Perks & Benefits

• People-work with talented, committed, and supportive teammates within an organization that values each member of its community.

• A meaningful place to grow and learn-whether it's your professional skills or scientific knowledge, we have the resources and environment to advance either so you can better support Gladstone's mission to drive a new era of discovery in disease-oriented science and to mentor tomorrow's leaders in an inspiring and diverse environment.

• Healthy work/life balance-you are highly engaged and productive at work because you can have time to recharge and enjoy a vibrant life outside of work.

• Compensation-competitive salary. Title and salary will be commensurate with education and experience.

• Excellent benefits-generous medical, dental, vision, retirement plan, paid vacation, commuter benefits, access to free shuttle transportation.

Gladstone is committed to providing equal employment opportunities to all employees and applicants for employment without regard to race, color, sex, religion, national origin, ancestry, age, marital status, medical condition, physical or mental disability, veteran status, sexual orientation, or any other non-job related characteristic. We make all employment decisions so as to further this principle of equal employment.