Protein Production Associate
Job ID: req1469
Employee Type: exempt full-time
Facility: ATRF: Other
Location: 8560 Progress Drive, Frederick, MD 21701 USA
The Frederick National Laboratory is a Federally Funded Research and Development Center (FFRDC) sponsored by the National Cancer Institute (NCI) and operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.
Our core values of accountability, compassion, collaboration, dedication, integrity, and versatility serve as a guidepost for how we do our work every day in serving the public's interest.
This position description does not represent a current opening but may be used to identify candidates with skills and experience for positions within Leidos Biomedical Research that frequently become available. Candidates who express an interest may be considered for future positions at Leidos Biomedical Research.
The Cancer Research Technology Program (CRTP) develops and implements emerging technology, cancer biology expertise and research capabilities to accomplish NCI research objectives. The CRTP is an outward-facing, multi-disciplinary hub purposed to enable the external cancer research community. A major focus of the CRTP is the NCI RAS Initiative with the goal to discover new therapeutic interventions against RAS-driven cancers. In addition, the CRTP hosts the Nanotechnology Characterization Laboratory (NCL) which performs and standardizes preclinical efficacy and toxicity testing of nanoparticles intended for cancer therapeutics and diagnostics, and the Antibody Characterization Lab (ACL) which characterizes monoclonal antibodies or other renewable affinity binding reagents for use in cancer related research. CRTP scientists also work collaboratively with intramural NCI investigators to provide research technologies and expertise.
The Protein Expression Laboratory (PEL) is standing up a new unit to produce high quality protein reagents for intramural NIH COVID-19 research efforts. The PEL creates expression constructs, expresses the encoded recombinant proteins in multiple expression systems, and purifies the recombinant proteins for use in a wide variety of applications including development and deployment of serology assays, structural biology, drug discovery, and biochemical analysis. The Protein Expression Associate will:
Carry out experiments, under the review of a scientist, in the areas of prokaryotic and eukaryotic protein production
Implement molecular biology processes for the generation of purified proteins
Carry out eukaryotic cell culture in insect and mammalian cells
Carry out bacterial fermentation using shake flask and bioreactor production
Perform microscale protein purification scouting and cell lysis procedures
Generate large-scale purified proteins using FPLC technology primarily using affinity and size-exclusion chromatography
Carry out quality control processes to validate the correctness of protein reagents
Troubleshoot current processes within the laboratory with an eye towards process improvement
Provide timely updates of project progress to supervisor and other staff in both informal and formal reports
Maintain detailed records of all laboratory processes and procedures for quality assurance purposes
Possession of a Bachelor's degree from an accredited college or university according to the Council for Higher Education Accreditation. Foreign degrees must be evaluated for U.S. equivalency.
Minimum of 2 years of relevant experience in molecular biology, biochemistry, or related disciplines
Previous experience in protein expression from prokaryotic or eukaryotic cells
Previous experience in protein purification
Must be able to obtain and maintain a clearance.
Cell culture experience with HEK293 or insect cells
Experience using bioreactors for prokaryotic protein production
Large-scale purification experience using FPLC technology
Ability to handle multiple scientific projects with a high degree of accuracy
Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)
Frederick National Laboratory