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Post-Baccalaureate Fellow (Nci)

Expired Job

Leidos Frederick , MD 21705

Posted 2 months ago

Description

PROGRAM DESCRIPTION

The Cancer Research Technology Program (CRTP)develops and implements emerging technology, cancer biology expertise andresearch capabilities to accomplish NCI research objectives. The CRTP is anoutward-facing, multi-disciplinary hub purposed to enable the external cancerresearch community. CRTP has two major focus areas, the NCI RAS Initiative withthe goal to discover new therapeutic interventions against RAS-driven cancersand to provide CCR and DCEG with multidisciplinary technology expertise neededfor address the complex etiology of human cancer. In addition, the CRTP hoststhe Nanotechnology Characterization Laboratory (NCL) which performs andstandardizes preclinical efficacy and toxicity testing of nanoparticlesintended for cancer therapeutics and diagnostics, and the AntibodyCharacterization Lab (ACL) which characterizes monoclonal antibodies or otherrenewable affinity binding reagents for use in cancer related research.

KEYROLES/RESPONSIBILITIES

The Protein Characterization Laboratory (PCL)provides support to the NCI CCR and DCEG in areas of protein characterizationand proteomics, macromolecular interaction and targeted and global metaboliteanalysis using variety of separation techniques and state of the art massspectrometry.

The Post-Baccalaureate Fellow will:

  • Develop new technologies for improvedidentification of HLA peptides, crucial for cancer vaccine development

  • Perform transient transfection in tissueculture cell to evaluate HLA peptide generation following over expression ofspecific target proteins

  • Generate stable cell lines, over expressingboth wild type and mutant form of specific test proteins, following evaluationof HLA peptide isolation efficiency

  • Perform large scale purification of HLA boundpeptides and develop methods to improve immunoprecipitation of HLA receptors

  • Large scale purification of HLA antibodies forimmunoprecipitation

  • Work with senior scientist to systematicallyexplore methods to improve ionization of HLA peptides

  • Acquire mass spectrometry data followingenhanced ionization and affinity purification of HLA peptides

  • Raw mass spectrometry data processing usingnovel data processing tools

  • Provide timely updates of project progress tosupervisor and other staff in both informal and formal reports

  • Maintain detailed records of all laboratoryprocesses and procedures for quality assurance purposes

  • Work in a team-oriented environment to carryout technology development efforts

The position isfunded for a period of one year.

Qualifications

BASIC QUALIFICATIONS

To be considered forthis position, you must minimally meet the knowledge, skills, and abilitieslisted below:

  • Possession of a Bachelor's degree from anaccredited college/university according to the Council for Higher EducationAccreditation (CHEA) in a field related to biomedical research. (Additionalqualifying experience may be substituted for the required education). Foreigndegrees must be evaluated for U.S. equivalency.

  • The Postbac Program is for individuals who meetany ONE of the following criteria:

  • College graduates who received their bachelor'sdegree less than THREE years prior to the date they begin the program,

  • Individuals who are more than 3 years past thereceipt of their bachelor's degree but received a master's degree less than SIXMONTHS before they begin the program, OR

  • Students who have been accepted into graduate,other doctoral, or medical school programs and who have written permission fromtheir school to delay entrance for up to one year to pursue a biomedicalresearch project at the NIH

  • Ability to follow and apply standard laboratoryproduction protocols and procedures

  • Abilityto lift and carry up to 25 lbs.; position requires standing, rapidmental/muscular coordination, good vision, and working alone or closely withothers

  • Must be able to obtain and maintain a securityclearance

PREFERREDQUALIFICATIONS

Candidates with thesedesired skills will be given preferential consideration:

  • Experience with eukaryotic cell culturetechniques

  • Experience with basic molecular biology skills(immunoprecipitation, western blotting, sample preparation for mass spectrometry)

  • Basic understanding of mass spectrometry anddata processing

  • Ability to handle multiple scientific projectswith a high degree of accuracy

  • Capacity to communicate effectively orally andin writing

Leidos is a global science and technology solutions leader working to solve the world's toughest challenges in the defense, intelligence, homeland security, civil, and health markets. The company's 33,000 employees support vital missions for government and commercial customers. Headquartered in Reston, Virginia, Leidos reported pro forma annual revenues of approximately $10 billion for the fiscal year ended January 1, 2016 after giving effect to the recently completed combination of Leidos with Lockheed Martin's Information Systems & Global Solutions business (IS&GS). For more information, visit www.Leidos.com. The company's diverse employees support vital missions for government and commercial customers. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability or veteran status. Leidos will consider qualified applicants with criminal histories for employment in accordance with relevant Laws. Leidos is an equal opportunity employer/disability/vet.


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Post-Baccalaureate Fellow (Nci)

Expired Job

Leidos