Molecular Technologist

Alakaina Family Of Companies Frederick , MD 21705

Posted 3 weeks ago

Laulima Government Solutions is looking for a qualified Molecular Technologist to provide Scientific Support at the National Institute of Allergies and Infectious Disease (NIAID) high containment facility.

The Molecular Research Associate will support government-approved research by providing technical support to characterize host-pathogen interactions and develop medical countermeasures to high consequence pathogens. The technician will perform and analyze microbiology and virology based assays. He/she will be responsible for proficiency in the use and maintenance of all equipment and procedures for the assigned area with ability to cross-train in related support areas. Duties include:

  • Work in major molecular research areas:

  • Structure and function of the mucosal immune system in the gastrointestinal system

  • Basic properties of neutrophils, nave and memory T cells, macrophages, and dendritic cells

  • Genetic and epigenetic regulation of chemokine receptor expression

  • Chemokines as mediators in antimicrobial host defense, inflammation, and cancer

  • Primary immunodeficiency disease

  • Virology assays to include but not limited to titration by TCID50 and plaque assays

  • DNA/RNA extraction, purification, quantification, transcription, replication, amplification, and mutagenesis, PCR/real-time PCR, microarray

  • Analysis of viral load in blood and tissue samples using quantitative real time PCR (one-step and two-step assays)

  • QC analysis of viral samples including endotoxin, mycoplasma, and sterility testing.

  • Implement assays per well-documented standards and contribute to the development of standard methods

  • Effectively manage tasks and can rapidly adjust to changing experimental priorities

  • Routinely work in high containment laboratories

  • Support foreign research activities; requires 3-4 week deployments to foreign locations on a rotational basis

  • General lab safety, organization, and maintenance

  • Maintain freezer and reagent inventories, common lab stocks, and lab equipment

  • Prepare packages for shipment

  • Maintain PCR primer, probe, and standards inventories

  • Prepare analyzed data

  • Participate in general laboratory housekeeping, to include biohazard waste management

  • Participate in a wide range of research facilitation, laboratory studies, analyses, analytic and computational models, simulations, and other technical and analytical support.

  • Engage in typical studies that entail exposure of laboratory rodents or non-human primates to microbial agents by appropriate routes (e.g. intravenous, intramuscular, or via aerosol) to elucidate an acute disease process or evaluate a therapeutic intervention.

  • Engage in longer term studies that include evaluation of candidate vaccines for immunogenicity and protective efficacy as well as evaluation of sequelae following recovery from acute disease. The isolation and characterization of previously unknown microbial agents may also be required. The disease processes shall be monitored using tools available in hospitals and clinics, especially imaging, telemetry, and clinical chemistry and hematology, in addition to the research tools of bacteriology, virology, immunology and pathology.

  • Generate study plans using approved templates and SharePoint processes

  • Comply with the NIH Division of Occupational Health and Safety requirement for satisfactory completion of the Biosurety program and Select Agent program.

  • Play a role in ensuring that the Biosurety program promotes a security conscious culture.

  • Comply with the physical security, protection of the division's property, protection of information, cyber security, agent protection, control and accountability, safe disposal and waste handling, and personal security guidelines set forth by the division's Biosurety program.

Degree/Education/Certification Requirements:
  • Bachelor of Science or higher degree. Biology and/or Chemistry preffered

  • Ability to work in BSL-4 setting

Required Skills and Experience:

  • Must be a US citizen or permanent resident.

  • The candidate must be willing to deploy overseas for short term assignments

  • Ability to obtain/maintain DoJ Security Risk Assessment (SRA) approval.

  • Must be willing to be immunized with licensed and FDA approved Investigational New Drugs (IND) recommended for persons at risk to occupational exposure of biological agents

  • Must be willing to participate in periodic drug screening.

  • Minimum of 2 years of applicable experience

  • Previous laboratory experience including basic understanding of design, optimization and implementation of quantitative PCR assays

  • Must be willing to work in a biosafety level 2 (BSL2) and/or 3 (BSL3) and/or 4 (BSL 4) environment and able to wear appropriate respiratory protection. The candidate will be required to work in BSL4 containment environment and will be required to pass physical exams and participate in vaccination programs.

  • Must be willing to be immunized with licensed and FDA approved Investigational New Drugs (IND) recommended for persons at risk to occupational exposure of biological agents.

  • Must demonstrate a willingness to participate in deployment rotations.

  • Individual must be able to meet the requirements for Public Trust Level 5b. For this level, candidates must complete and pass a National Agency Check with Inquiries (NACI) with fingerprinting, and a Minimum Background Investigation (MBI).

  • Must have the ability to obtain/maintain certification in Chemical Personnel Reliability Program and/or Biological Personnel Reliability Program.

  • Must have the ability to obtain/maintain CDC Select Agent Program approval.

  • Willingness to work under BSL-3 - BSL-4 conditions. Able to wear respiratory protection.

Desired Skills and Experience :

  • Previous field work experience.

  • Computer Skills:

  • Proficiency in Word, Excel, and PowerPoint

  • Proficiency in ABI 7500 Dx Real Time PCR Acquisition and Analysis software

  • Proficiency in Primer/Probe design software such as Primer Express or Seqbuilder.

  • Experience in preparing graphs and figures for publication using Excel and Graphpad Prism.

  • Basic understanding of laboratory information management systems (LIMS)

  • Experience with viral Select Agents is highly desired

  • Experience in BSL-3 or BSL-4 containment

  • Good Laboratory Practice experience is preferred.

  • Experience in the development, evaluation, verification, and validation of molecular diagnostic assays.

  • Demonstrated ability to work independently, design experiments and protocols, and analyze data.

  • Demonstrated critical thinking skills/problem solving/good reasoning skills

  • Demonstrated experience preparing and reviewing scientific presentations and manuscripts.

  • Expected to participate in any required vaccination programs.

  • Excellent customer relations and interpersonal skills required.

  • Excellent oral and written communication and organizational skills.

  • Knowledge of Imaging Modalities to include: MRI, PET, SPECT and X-Ray

  • Demonstrate interpersonal, oral and written communication, and organizational skills,

  • Demonstrate ability to ability to work independently, design experiments, and analyze data

  • Familiarity with hospital tools such as endoscopy, cardiac telemetry monitors, and Computed Tomography (CT), Magnetic Resonance Imaging (MRI), Single Positron Emission Computed Tomography (SPECT), and Positron Emission Tomography (PET) and Ultrasonography imaging to systematically evaluate the pathogenic processes and clinical course of disease in animal models exposed to microbes.

Clearance:

  • Individual must be able to meet the requirements for Public Trust Level 5b. For this level, candidates must complete and pass a National Agency Check with Inquiries (NACI) with fingerprinting, and a Minimum Background Investigation (MBI).

Laulima Government Solutions, LLC is a fast-growing government service provider. Employees enjoy competitive salaries; a 401K plan with company match; medical, dental, disability, and life insurance coverage; tuition reimbursement; paid time off; and 10 paid holidays. Laulima Government Solutions, LLC is proud to be an equal opportunity employer.

We are an Equal Opportunity/Affirmative Action Employer of individuals with disabilities and veterans. We are proud to state that we do not discriminate in employment decisions on the basis of race, color, religion, sex, pregnancy, sexual orientation, gender identity, national origin, age, protected veteran status, or disability status. If you are a person with a disability and you need an accommodation during the application process, please click here to request accommodation. We E-Verify all employees.

The Alakaina Foundation Family of Companies (FOCs) is comprised of industry-recognized government service firms designated as Native Hawaiian Organization (NHO)-owned and 8(a) certified businesses. The Family of Companies (FOCs) includes Keaki Technologies, Laulima Government Solutions, Kpono Government Services, and Kapili Services, Pookela Solutions, Kkaha Solutions, LLC, and Pololei Solutions, LLC. Alakaina Foundation activities under the 501(c)3 principally benefit the youth of Hawaii through charitable efforts which includes providing innovative educational programs that combine leadership, science & technology, and environmental stewardship.

For additional information, please visit www.alakainafoundation.com

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Laulima Government Solutions, LLC is a fast-growing government service provider. Employees enjoy competitive salaries; a 401K plan with company match; medical, dental, disability, and life insurance coverage; tuition reimbursement; paid time off; and 10 paid holidays. Laulima Government Solutions, LLC is proud to be an equal opportunity employer. We are an Equal Opportunity/Affirmative Action Employer of individuals with disabilities and veterans. We are proud to state that we do not discriminate in employment decisions on the basis of race, color, religion, sex, pregnancy, sexual orientation, gender identity, national origin, age, protected veteran status, or disability status. If you are a person with a disability and you need an accommodation during the application process, please click here to request accommodation. We E-Verify all employees. The Alaka`ina Foundation Family of Companies (FOCs) is comprised of industry-recognized government service firms designated as Native Hawaiian Organization (NHO)-owned and 8(a) certified businesses. The Family of Companies (FOCs) includes Ke`aki Technologies, Laulima Government Solutions, Kūpono Government Services, and Kapili Services, Po`okela Solutions, Kīkaha Solutions, LLC, and Pololei Solutions, LLC. Alaka`ina Foundation activities under the 501(c)3 principally benefit the youth of Hawaii through charitable efforts which includes providing innovative educational programs that combine leadership, science & technology, and environmental stewardship. For additional information, please visit www.alakainafoundation.com #Monster #Clearancejobs Alakaina Family Of Companies Frederick MD

Molecular Technologist

Alakaina Family Of Companies