Located in a historic neighborhood in the nation's capital, Georgetown offers rigorous academic programs, a global perspective, exciting ways to take advantage of Washington, D.C., and a commitment to social justice. Our community is a tight knit group of remarkable individuals interested in intellectual inquiry and making a difference in the world.
The Laboratory Technician is responsible for carrying out experiments related to cancer research projects, especially generation and maintenance of patient-derived conditionally reprogrammed cell lines and 3D primary cell cultures from various tumor tissue types. S/he maintains primary cell cultures directly from patient's tissue materials as well as from mouse PDX tissue materials. The incumbent works with Dr. Agarwal's research group, with their work helping to move the goals and helping in securing additional funding to carry out the research in the Center and in the Department of Pathology. Supervised directly by Dr. Sujata Choudhury in the lab, the Laboratory Technician has additional duties that include but are not limited to:
Establish primary cell cultures using patient's or mouse PDX tissue materials using conditionally reprogram cell technology.
Maintain the cultures and carry out proliferation assays, checking the presence of mouse cells in the cultures to see if they are derived from PDX tissue materials using PCR.
Carry out several molecular biology techniques, such as DNA and RNA isolation from cells and tissue materials, qPCR, and gel electrophoresis for DNA and proteins.
Prepare protein cell lysates from cells and Western Blotting, as well asImmunofluorescence staining of cells, capturing images.
Participate in writing paper, making figures ready for presentations and publications.
Write day to day experiments in detail in the lab note book, and provide data analysis using excel and statistical software.
Conduct lab chores including keeping lab clean, washing glassware, and autoclaving.
Isolate cells from zebrafish and expand them using CR cell culture conditions.
Image zebrafish using IF microscope and other duties as assigned.
Requirements and Qualifications
A Bachelor's of Science: Master's preferred
At least two years of experience in tissue culture, DNA/RNA isolation, PCR, protein gels, immunoblotting
Working knowledge of immunofluorescence microscopy
Working knowledge of Microsoft word, excel, photoshop, powerpoint and any statistical analysis software
Any experience with zebrafish handling and injections in embryos will be a plus
Current Georgetown Employees:
If you currently work at Georgetown University, please exit this website and login to GMS (gms.georgetown.edu) using your Net ID and password. Then select the Career worklet on your GMS Home dashboard to view Jobs at Georgetown.
Please note that in order to be considered an applicant for any position at Georgetown University you must submit a cover letter and resume for each position of interest for which you believe you are qualified. These documents are not kept on file for future positions.
If you are a qualified individual with a disability and need a reasonable accommodation for any part of the application and hiring process, please click here for more information, or contact the Office of Institutional Diversity, Equity, and Affirmative Action (IDEAA) at 202-687-4798 or firstname.lastname@example.org.
Need some assistance with the application process? Please call 202-687-2500. For more information about the suite of benefits, professional development and community involvement opportunities that make up Georgetown's commitment to its employees, please visit the Georgetown Works website.
Georgetown University is an Equal Opportunity/Affirmative Action Employer fully dedicated to achieving a diverse faculty and staff. All qualified applicants are encouraged to apply and will receive consideration for employment without regard to race, color, religion, national origin, age, sex (including pregnancy, gender identity and expression, and sexual orientation), disability status, protected veteran status, or any other characteristic protected by law.
Georgetown University offers a wide variety of comprehensive and competitive benefits. Benefits packages include comprehensive health, dental and vision plans, disability and life insurance coverage, retirement savings programs, tuition assistance, voluntary insurance options (including group legal, accident, and critical illness), and much more. Whatever your need, the Office of Faculty and Staff Benefits will be standing by to support you. You can learn more about the benefits offered to eligible faculty and staff at https://benefits.georgetown.edu or view the online interactive benefits guide for more information.