The Fluid Biomarkers group (Section of Clinical Sciences) is seeking a creative and collaborative lab-based scientist engaged in targeted proteomics for the detection of proteins and metabolites in different biological matrices (including plasma, serum, CSF, urine, tissues). This role is required to develop and implement clinically robust LC-MS assays to support clinical programs across the Neurology portfolio. The successful candidate will be capable of multitasking and effective planning to consistently meet deadlines.
Targeted protein biomarker assay development using state of the art liquid-chromatography-mass spectrometry (LC-MS) platforms including nano-LC, orbitrap (Q-Exactive, Q-Exactive +, Q-Exactive HF/HFX, Fusion) and UPLC-QQQ MS (AB Sciex)
Sample preparation (tissues and cell lysates) for protein quantification
Biotinylation and various bead-antibody conjugation techniques
Identification and selection of antibodies for immunoprecipitation prior to LC-MS analysis
Identification of novel techniques and strategies to enhance assay sensitivity
Fit for purpose qualification of protein and small molecule LC-MS assays
LC-MS instrumentation troubleshooting
Point of contact with Contract Research Organizations
Work with CRO partners to oversee biomarker assay implementation including assay interpretation
Timely recording keeping and data entry into Electronic Lab Notebook
Prepare, review, and deliver scientific presentations for internal/external use
Proven experience with LC-MS instrumentation is required with experience in targeted and global proteomic profiling.
Preference will be given to candidates with hands on experience in developing and qualifying high resolution (Orbitrap) LC-MS assays, including immunoprecipitation LC-MS for the detection of proteins in biological samples
Expertise using Skyline and familiarity with Thermo Xcalibur, Proteome Discoverer, AB Sciex Analyst
Excellent assay troubleshooting skills
Familiarity with neuroscience, including knowledge of neurological indications such as AD, PD, ALS, or MS is considered a plus but not required
Experience in or willingness to learn classic immunoassay based assays (ELISA)
Demonstrated track record of providing support activities for multiple research projects
Solid work ethic and demonstrated record of productivity such as participating in peer reviewed publications or scientific poster presentations
All your information will be kept confidential according to EEO guidelines.
The Fluid Biomarkers group is seeking a creative and collaborative lab-based scientist engaged in targeted proteomics for the detection of proteins and metabolites in different biological matrices (including plasma, serum, CSF, urine, tissues). This role is required to develop and implement clinically robust LC-MS assays to support clinical programs across the Neurology portfolio.